The 5'-7-methylguanosine cap on eukaryotic mRNAs serves both to stimulate canonical translation initiation and to block an alternative pathway.
Mitchell SF, Walker SE, Algire MA, Park EH, Hinnebusch AG, Lorsch JR
Mol Cell. 2010 Sep 24;39(6):950-62.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
37223 | pUC19-RPL41A | |
37224 | pUC19-DAD4 | |
37225 | pTYB1-eIF4A | |
37226 | pTYB2-eIF4B | |
37227 | pTYB2-eIF4B-A130C | |
37228 | pET15b-eIF4E | |
37229 | pET15b-eIF4E-S200C | |
37230 | pET21c-eIF4A | |
37231 | pGEX4T1-GST-eIF4G1-His6 | |
37232 | pET-DUET-GST-eIF4G1-His6, eIF4E | |
37233 | pTYB2-HCR1 |