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A bimolecular affinity purification method under denaturing conditions for rapid isolation of a ubiquitinated protein for mass spectrometry analysis.

Maine GN, Li H, Zaidi IW, Basrur V, Elenitoba-Johnson KS, Burstein E
Nat Protoc. 2010 Aug;5(8):1447-59. Epub 2010 Jul 22. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
36098pEBB-BT-Ubiquitin
36099pEBB-TB
36295pEBB-BT

Antibodies from Article