A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells.
Roux KJ, Kim DI, Raida M, Burke B
J Cell Biol. 2012 Mar 12.
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PubMed
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Article
Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
35700 | pcDNA3.1 mycBioID | To fuse your protein of interest to the C-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); myc tag |
36047 | pcDNA3.1 MCS-BirA(R118G)-HA | To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); HA tag |
80901 | mycBioID-pBABE-puro | To fuse your protein of interest to the C-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); myc tag |