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A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells.

Roux KJ, Kim DI, Raida M, Burke B
J Cell Biol. 2012 Mar 12. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
35700pcDNA3.1 mycBioIDTo fuse your protein of interest to the C-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); myc tag
36047pcDNA3.1 MCS-BirA(R118G)-HATo fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); HA tag
80901mycBioID-pBABE-puroTo fuse your protein of interest to the C-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); myc tag

Antibodies from Article