Controlled gene expression in primary Lgr5 organoid cultures.
Koo BK, Stange DE, Sato T, Karthaus W, Farin HF, Huch M, van Es JH, Clevers H
Nat Methods. 2011 Dec 4. doi: 10.1038/nmeth.1802.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 32702 | pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE | Conditional overexpression vector. Deletion of dsRed by Cre recombinase results in the rapid loss of dsRed and the activation of your gene fused to eGFP expression. |
| 32703 | pMSCV-loxp-dsRed-loxp-3xHA-Puro-WPRE | |
| 32704 | pMSCV-FLIP-puro-dsRed-GFP-miRNA |