mTORC1 senses lysosomal amino acids through an inside-out mechanism that requires the vacuolar H-ATPase.
Zoncu R, Bar-Peled L, Efeyan A, Wang S, Sancak Y, Sabatini DM
Science. 2011 Nov 4;334(6056):678-83.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 34611 | LAMP1-mRFP-FLAG | 3rd gen lentiviral vector expressing a fusion protein of Lamp1, mRFP, and a FLAG tag |
| 87931 | pRK5-FLAG-ATP6V0D1 | FLAG-tagged overexpression |
| 87932 | pRK5-ATP6V1D-HAGST | C-term HA-GST tagged overexpression |
| 88856 | pRK5-myc-PAT1 | myc-TAG overexpression |