A system for the construction of targeted unmarked gene deletions in the genus Burkholderia.
Flannagan RS, Linn T, Valvano MA
Environ Microbiol. 2008 Jun;10(6):1652-60. Epub 2008 Mar 13.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 32060 | pGPI-SceI | Construction of unmarked gene deletions. Suicide cloning vector designed to introduce a targeted I-SceI restriction site in the genome of Burkholderia |
| 32061 | pDAI-SceI | Construction of unmarked gene deletions. Broad host range replicative plasmid expressing the I-SceI homing endonuclease |