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Alternative splicing regulates mouse embryonic stem cell pluripotency and differentiation.

Salomonis N, Schlieve CR, Pereira L, Wahlquist C, Colas A, Zambon AC, Vranizan K, Spindler MJ, Pico AR, Cline MS, Clark TA, Williams A, Blume JE, Samal E, Mercola M, Merrill BJ, Conklin BR
Proc Natl Acad Sci U S A. 2010 Jun 8;107(23):10514-9. Epub 2010 May 24. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
31845pSicoR-Ef1a-mCh-PuroCre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression. Includes puromycin selection.
31847pSicoR-Ef1a-mChCre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression.
31848pSicoR-Ef1a-mCh-Puro-GFPi
31849pSicoR-Ef1a-mCh-GFPi
31850pSicoR-Ef1a-mCh-Puro-Luci

Antibodies from Article