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An improved cerulean fluorescent protein with enhanced brightness and reduced reversible photoswitching.

Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, Day RN, Wachter RM, Davidson MW, Rizzo MA
PLoS One. 2011 . 6(3):e17896. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
54605mCerulean3-C1Localization: C1 Cloning Vector, Excitation: 433, Emission: 475
54666mCerulean-pBAD
54721mCerulean3-Lifeact-7Localization: Actin, Excitation: 433, Emission: 475
54730mCerulean3-N1Localization: N1 Cloning Vector, Excitation: 433, Emission: 475
55399mCerulean3-Actin-7Localization: Actin, Excitation: 433, Emission: 475
55400mCerulean3-Actin-C-18Localization: Actin, Excitation: 433, Emission: 475
55401mCerulean3-Alpha-5-Integrin-12Localization: Focal Adhesions, Excitation: 433, Emission: 475
55403mCerulean3-CAF1-C-10Localization: Chromatin Assembly Factor 1, Excitation: 433, Emission: 475
55404mCerulean3-Caveolin-C-10Localization: Membrane, Excitation: 433, Emission: 475
55405mCerulean3-CD36-C-10Localization: Membrane, Excitation: 433, Emission: 475
55406mCerulean3-CDC42-C-10Localization: Membrane, Excitation: 433, Emission: 475
55407mCerulean3-CEBPA237-C-4Localization: Nucleus, Excitation: 433, Emission: 475
55408mCerulean3-Clathrin-15Localization: Clathrin Vesicles, Excitation: 433, Emission: 475
55409mCerulean3-C-Src-7Localization: Membrane/Vesicles, Excitation: 433, Emission: 475
55410mCerulean3-Cx26-7Localization: Gap Junctions, Excitation: 433, Emission: 475
55411mCerulean3-Cx43-7Localization: Gap Junctions, Excitation: 433, Emission: 475
55412mCerulean3-CytERM-N-17Localization: Endoplasmic Reticulum, Excitation: 433, Emission: 475
55413mCerulean3-Dectin1A-C-10Localization: Membrane, Excitation: 433, Emission: 475
55414mCerulean3-Dectin1A-N-10Localization: Membrane, Excitation: 433, Emission: 475
55415mCerulean3-EB1-C-18Localization: MT End Binding Protein, Excitation: 433, Emission: 475
55416mCerulean3-EB3-7Localization: MT End Binding Protein, Excitation: 433, Emission: 475
55417mCerulean3-EB3-C-20Localization: MT End Binding Protein, Excitation: 433, Emission: 475
55418mCerulean3-ER-5Localization: Endoplasmic Reticulum, Excitation: 433, Emission: 475
55419mCerulean3-Fibrillarin-7Localization: Nucleoli, Excitation: 433, Emission: 475
55420mCerulean3-Golgi-7Localization: GalT Targeting Sequence, Excitation: 433, Emission: 475
55421mCerulean3-H2B-6Localization: Nucleus/Histones, Excitation: 433, Emission: 475
55422mCerulean3-H2B-C-10Localization: Nucleus/Histones, Excitation: 433, Emission: 475
55423mCerulean3-Integrin-Beta1-N-18Localization: Integrin/Focal Adhesions, Excitation: 433, Emission: 475
55424mCerulean3-Keratin-17Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 433, Emission: 475
55425mCerulean3-LaminB1-10Localization: Nuclear Envelope, Excitation: 433, Emission: 475
55426mCerulean3-Lasp1-N-10Localization: Focal Adhesions, Excitation: 433, Emission: 475
55427mCerulean3-LC-Myosin-C-10Localization: Light Chain Myosin, Excitation: 433, Emission: 475
55428mCerulean3-Lysosomes-20Localization: Lysosome Membrane, Excitation: 433, Emission: 475
55429mCerulean3-MannII-N-10Localization: Mannosidase/Golgi, Excitation: 433, Emission: 475
55431mCerulean3-MAPTau-C-10Localization: Microtubules, Excitation: 433, Emission: 475
55432mCerulean3-MCHR1-N-10Localization: Cilium, Excitation: 433, Emission: 475
55433mCerulean3-Mito-7Localization: Mitochondria, Excitation: 433, Emission: 475
55434mCerulean3-Myotilin-C-14Localization: Cytoskeleton, Excitation: 433, Emission: 475
55435mCerulean3-Palladin-C-7Localization: Cytoskeleton, Excitation: 433, Emission: 475
55436mCerulean3-Paxillin-22Localization: Focal Adhesions, Excitation: 433, Emission: 475
55437mCerulean3-PCNA-19-SV40NLS-4Localization: Nucleus, Excitation: 433, Emission: 475
55438mCerulean3-Plastin-C-10Localization: Fimbrin/Cytoskeleton, Excitation: 433, Emission: 475
55439mCerulean3-PMP-N-10Localization: Peroxisomes, Excitation: 433, Emission: 475
55440mCerulean3-Profilin-10Localization: Cytoskeleton, Excitation: 433, Emission: 475
55441mCerulean3-PTK2-C-10Localization: FAK/Focal Adhesions, Excitation: 433, Emission: 475
55442mCerulean3-PTK2-N-10Localization: FAK/Focal Adhesions, Excitation: 433, Emission: 475
55443mCerulean3-Rab4a-7Localization: Ras GTPase, Excitation: 433, Emission: 475
55444mCerulean3-Rab5a-7Localization: Ras GTPase, Excitation: 433, Emission: 475
55445mCerulean3-SiT-N-15Localization: Golgi TGN, Excitation: 433, Emission: 475
55446mCerulean3-SSTR3-N-17Localization: Cilium, Excitation: 433, Emission: 475
55447mCerulean3-Talin-18Localization: Focal Adhesions, Excitation: 433, Emission: 475
55448mCerulean3-Talin-N-10Localization: Focal Adhesions, Excitation: 433, Emission: 475
55449mCerulean3-TOMM20-N-10Localization: Outer Mitochondrial Membrane, Excitation: 433, Emission: 475
55450mCerulean3-Tubulin-C-18Localization: Microtubules, Excitation: 433, Emission: 475
55452mCerulean3-Vimentin-7Localization: Intermediate Filaments, Excitation: 433, Emission: 475
55453mCerulean3-Vinculin-23Localization: Focal Adhesions, Excitation: 433, Emission: 475
55454mCerulean3-Zyxin-6Localization: Focal Adhesions, Excitation: 433, Emission: 475

Antibodies from Article