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A flow cytometric method to detect protein-protein interaction in living cells by directly visualizing donor fluorophore quenching during CFP-->YFP fluorescence resonance energy transfer (FRET).

He L, Olson DP, Wu X, Karpova TS, McNally JG, Lipsky PE
Cytometry A. 2003 Oct . 55(2):71-85. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
24520pCMV-ECFPEYFP

Antibodies from Article