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Ubiquitin ligase Nedd4L targets activated Smad2/3 to limit TGF-beta signaling.

Gao S, Alarcon C, Sapkota G, Rahman S, Chen PY, Goerner N, Macias MJ, Erdjument-Bromage H, Tempst P, Massague J
Mol Cell. 2009 Nov 13. 36(3):457-68. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
26996pCI Flag Smad3 LC WT
26997pCS2 Flag Smad3 T179V
26998pCS2 Flag Smad3 EPSM V179T
26999pCI HA NEDD4 DD
27000pCI HA NEDD4L
27001pCI HA NEDD4L DD
27002pCI HA NEDD4
27003pCI HA NEDD4L S382A S468A
27004pCS2 Flag Smad3 AAGY
27005pCI Flag Smad3 NL* EPSM
27006pCI Flag Smad3 NL EPSM
27010pGEX6-Smad3 WT
27011pGEX6-Smad3 T179V
27013pGEX6-Smad3 AAGY
27015pGEX6-Smad3 EPSM V179T
27016pRS-puro shNEDD4L #1
27017pRS-puro shNEDD4L #2
27025pQCXIH-Flag Smad3 WT
27026pQCXIH-Flag Smad3 EPSM
27027pQCXIH-Flag Smad3 AAGY
27028pQCXIH-HA NEDD4L
27036pCI HA NEDD4L S382A
27037pCI HA NEDD4L S468A
27038pCI Flag Smad3 LC EPSM
27039pCI Flag Smad3 LC AAGY
27040pCI Flag Smad3 NL WT
27041pCI Flag Smad3 NL AAGY
27042pCI Flag Smad3 NL* WT
27108pGEX6-Smad3 EPSM A204S
27109pGEX6-Smad3 EPSM A208S
27110pGEX6-Smad3 EPSM A213S
27111pCS2 Flag Smad3 EPSM A204S
27112pCS2 Flag Smad3 EPSM A208S
27113pCS2 Flag Smad3 EPSM A213S
27114pCS2 Flag Smad3 S204A
27115pCS2 Flag Smad3 S208A
27116pCS2 Flag Smad3 S213A
27222pGEX6-Smad3 EPSM

Antibodies from Article