The FKBP12-rapamycin-binding domain is required for FKBP12-rapamycin-associated protein kinase activity and G1 progression.
Vilella-Bach M, Nuzzi P, Fang Y, Chen J
J Biol Chem. 1999 Feb 12. 274(7):4266-72.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 26603 | pcDNA3-Flag mTOR wt | Mammalian expression of human flag-tagged mTor |
| 26604 | pcDNA3-Flag mTOR S2035T | |
| 26605 | pcDNA3-Flag mTOR S2035T D2357E | |
| 26606 | pcDNA3-Flag mTOR S2035T R2109A | |
| 26607 | pGEX-2T FRB | |
| 26608 | pGEX-2T FRB S2035I | |
| 26609 | pGEX-2T FRB R2109A | |
| 68726 | pGEX-2T FRB Y2105A | Point mutation Tyr 2105 → Ala in the FKBP12-rapamycin binding (FRB) domain of mTOR |
| 73341 | pGEX-2T-FRB-W2027F | Point mutation Trp 2027 → Phe in the FKBP12-rapamycin binding (FRB) domain of mTOR |