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DCP1 forms asymmetric trimers to assemble into active mRNA decapping complexes in metazoa.

Tritschler F, Braun JE, Motz C, Igreja C, Haas G, Truffault V, Izaurralde E, Weichenrieder O
Proc Natl Acad Sci U S A. 2009 Dec 22. 106(51):21591-6. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
25030pT7-EGFP-C1-HsDCP1a
25031pT7-EGFP-C1-HsDCP2
25032pT7-EGFP-C1-HsEDC3
25033pT7-EGFP-C1-HsRCK
66597pCIneo-lambdaN-HA-HsEDC4Expresses lambdaN-HA-tagged EDC4 in mammalian cells
146400pCIneo-lambdaN-HA-HsDCP1a_GMammalian Expression of HsDcp1a
146401pCIneo-lambdaN-HA-HsDCP2_GMammalian Expression of HsDCP2
146402pRSFDuetG-DmDCP1_328-366 (MCSII)_GBacterial Expression of DmDCP1_328-366
146403pRSFDuetG-DmDCP1_328-366 (MCSII)-mut1_GBacterial Expression of DmDCP1_328-366-mut1
146404pRSFDuetG-DmDCP1_328-366 (MCSII)-mut2_GBacterial Expression of DmDCP1_328-366-mut2
146405pRSFDuetG-DmDCP1_328-366 (MCSII)-mut3_GBacterial Expression of DmDCP1_328-366-mut3
146406pRSFDuetG-DmDCP1_328-366 (MCSII)-WT_GBacterial Expression of DmDCP1_328-366
146407pRSFDuetG-HsDCP1a_539-582 (MCSII)_GBacterial Expression of HsDCP1a_539-582
146408pRSFDuetG-HsDCP1a_539-582 (MCSII)-mut1_GBacterial Expression of HsDCP1a_539-582-mut1
146409pRSFDuetG-HsDCP1a_539-582 (MCSII)-mut2_GBacterial Expression of HsDCP1a_539-582-mut2
146410pRSFDuetG-HsDCP1a_539-582 (MCSII)-mut3_GBacterial Expression of HsDCP1a_539-582-mut3
146411pRSFDuetG-HsDCP1a_539-582 (MCSII)-WT_GBacterial Expression of HsDCP1a_539-582
146412pT7-EGFP-C1-HsDCP1a_539-582_GMammalian Expression of HsDcp1a_539-582
146413pT7-EGFP-C1-HsDCP1a-del155-168_GMammalian Expression of HsDcp1a-del155-168
146414pT7-EGFP-C1-HsDCP1a-del539-582_GMammalian Expression of HsDcp1a-del539-582
146415pT7-EGFP-C1-HsDCP1a-mut1_GMammalian Expression of HsDCP1a-mut1
146416pT7-EGFP-C1-HsDCP1a-mut2_GMammalian Expression of HsDCP1a-mut2
146417pT7-EGFP-C1-HsDCP1a-mut3_GMammalian Expression of HsDCP1a-mut3
146500pAc5.1B-EGFP-DmDCP1_327-372_HInsect Expression of DmDCP1_327-372
146501pAc5.1B-HA-DmDCP1_1-327-dsRNAres_HInsect Expression of DmDCP1_1-327
146502pAc5.1B-HA-DmDCP1-dsRNAres_HInsect Expression of DmDCP1
146503pAc5.1B-lambdaN-HA-DmDCP1_327-372-GST-V5His6_HInsect Expression of DmDCP1_327-372
146504pCIneo-lambdaN-HA-HsDCP1a-withDmTD_HMammalian Expression of HsDcp1a-withDmTD
146505pLNHA-C1-HsDCP1b_HMammalian Expression of HsDCP1b
146506pT7-EGFP-C1-HsDCP1a-withDmTD_HMammalian Expression of HsDCP1a-withDmTD

Antibodies from Article