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Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases.

Hockemeyer D, Soldner F, Beard C, Gao Q, Mitalipova M, Dekelver RC, Katibah GE, Amora R, Boydston EA, Zeitler B, Meng X, Miller JC, Zhang L, Rebar EJ, Gregory PD, Urnov FD, Jaenisch R
Nat Biotechnol. 2009 Aug 13. ():. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
22072AAVS1 hPGK-PuroR-pA donor
22073TV-hPITX3-forward
22074pTRE-TIGHT-EGFP-donor fw copy
22075AAVS1 SA-2A-puro-pA donorAAV zinc finger donor vector for genomic targeting
22077TRE-TIGHT-EGFP-backward donor
22208TV-hPITX3-Reverse
22209OCT4 GFP puro Donor 1
22210OCT4 GFP puro Donor 2
22211OCT4 GFP puro Donor 3
22212AAV-CAGGS-EGFPAAV zinc finger donor vector for genomic targeting

Antibodies from Article