Structure and biochemistry-guided engineering of an all-RNA system for DNA insertion with R2 retrotransposons.

Edmonds KK, Wilkinson ME, Strebinger D, Chen H, Lash B, Schaefer CC, Zhu S, Liu D, Zilberzwige-Tal S, Ladha A, Walsh ML, Frangieh CJ, Vaz Reay NA, Macrae RK, Wang X, Zhang F
Nat Commun. 2025 Jul 2;16(1):6079. doi: 10.1038/s41467-025-61321-z. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
238105	pIVT-R2Tg-WT	For IVT of wildtype R2Tg editor, codon optimized for mammalian expression.
238106	pIVT-R2Tg-RTmut	For IVT of RT-mutant R2Tg editor, codon optimized for mammalian expression.
238107	pTmpl-miniDonor-GFP	Mini donor expressing GFP, used for PCR to generate IVT template.