Probing condensate microenvironments with a micropeptide killswitch.
Zhang Y, Stoppelkamp I, Fernandez-Pernas P, Allram M, Charman M, Magalhaes AP, Piedavent-Salomon M, Sommer G, Sung YC, Meyer K, Grams N, Halko E, Dongre S, Meierhofer D, Malszycki M, Ilik IA, Aktas T, Kraushar ML, Vastenhouw N, Weitzman MD, Grebien F, Niskanen H, Hnisz D
Nature. 2025 Jun 4. doi: 10.1038/s41586-025-09141-5.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 237619 | pRK5_msfGFP-HMGB1-Mut-FtoED | For overexpression of msfGFP-HMGB1-Mut-FtoED |
| 237620 | pRK5_msfGFP-HMGB1-Mut-FtoA | For overexpression of msfGFP-HMGB1-Mut-FtoA |
| 237621 | pRK5_msfGFP-HMGB1-Mut-FtoG | For overexpression of msfGFP-HMGB1-Mut-FtoG |
| 237622 | pRK5_msfGFP-HMGB1-Mut-0F | For overexpression of msfGFP-HMGB1-Mut-0F |
| 237623 | pRK5_msfGFP-HMGB1-Mut-F12A | For overexpression of msfGFP-HMGB1-Mut-F12A |
| 237624 | pRK5_msfGFP-HMGB1-Mut-F13A | For overexpression of msfGFP-HMGB1-Mut-F13A |
| 237625 | pRK5_msfGFP-HMGB1-Mut-deltaKS 3F | For overexpression of msfGFP-HMGB1-Mut-deltaKS 3F |
| 237626 | pRK5_msfGFP-HMGB1-Mut-11G3F3G | For overexpression of msfGFP-HMGB1-Mut-11G3F3G |
| 237627 | pRK5_msfGFP-HMGB1-Mut-C16toA | For overexpression of msfGFP-HMGB1-Mut-C16toA |
| 237628 | pRK5_msfGFP-HMGB1-Mut-MtoED | For overexpression of msfGFP-HMGB1-Mut-MtoED |
| 237629 | pET22b_msfGFP_stag | For expression of msfGFP_stag in Ecoli |
| 237630 | pET22b_msfGFP_stag_KS | For expression of msfGFP_stag_KS in Ecoli |
| 237631 | pET22b_msfGFP_stag_KS(FtoG) | For expression of msfGFP_stag_KS(FtoG) in Ecoli |
| 237632 | pUC19_msfGFP-TCOF1-repair-template | For knock-in msfGFP to TCOF1 locus |
| 237633 | pX458_mCherry-TCOF1-gRNA | Cas9 from S. pyogenes with 2A-mCherry, gRNA to knock-in msfGFP to TCOF1 locus |
| 237634 | pX458_mCherry-HP1a-gRNA-1 | Cas9 from S. pyogenes with 2A-mCherry, gRNA to knock-in msfGFP to HP1a locus |
| 237635 | pX458_mCherry-HP1a-gRNA-2 | Cas9 from S. pyogenes with 2A-mCherry, gRNA to knock-in msfGFP to HP1a locus |
| 237636 | pRK5_mEGFP-EWS-FLI1 | For overexpression of mEGFP-EWS-FLI1 |
| 237637 | pRK5_mEGFP-SS18-SSX2 | For overexpression of mEGFP-SS18-SSX2 |
| 237638 | pRK5_mEGFP-CRTC1-MAML2 | For overexpression of mEGFP-CRTC1-MAML2 |
| 237639 | pRK5_mEGFP-NUP98-HOXA9 | For overexpression of mEGFP-NUP98-HOXA9 |
| 237640 | pRK5_mEGFP-NUP98-DDX10 | For overexpression of mEGFP-NUP98-DDX10 |
| 237641 | pRK5_mEGFP-NONO-TFE3 | For overexpression of mEGFP-NONO-TFE3 |
| 237642 | pRK5_mEGFP-TAZ-CAMTA1 | For overexpression of mEGFP-TAZ-CAMTA1 |
| 237643 | pRK5_mEGFP-PAX-FOXO1 | For overexpression of mEGFP-PAX-FOXO1 |
| 237644 | pRK5_mEGFP-NUP98-DDX10-KS | For overexpression of mEGFP-NUP98-DDX10-KS |
| 237645 | pRK5_mEGFP-NUP98-DDX10-KS-FtoG | For overexpression of mEGFP-NUP98-DDX10-KS-FtoG |
| 237646 | pRK5_mCherry-CRM1 | For overexpression of mCherry-CRM1 |
| 237647 | pRK5_mEGFP-NUP98-DDX10-Mut1 | For overexpression of mEGFP-NUP98-DDX10-Mut1 |
| 237648 | pRK5_mEGFP-NUP98-DDX10-Mut2 | For overexpression of mEGFP-NUP98-DDX10-Mut2 |
| 237649 | pRK5_msfGFP-HMGB1-Shuffled 1 | For overexpression of msfGFP-HMGB1-Shuffled 1 |
| 237650 | pRK5_msfGFP-HMGB1-Shuffled 2 | For overexpression of msfGFP-HMGB1-Shuffled 2 |
| 237651 | pRK5_msfGFP-HMGB1-Shuffled 3 | For overexpression of msfGFP-HMGB1-Shuffled 3 |
| 237652 | pRK5_msfGFP-HMGB1-Shuffled 4 | For overexpression of msfGFP-HMGB1-Shuffled 4 |
| 237653 | pRK5_msfGFP-HMGB1-Shuffled 5 | For overexpression of msfGFP-HMGB1-Shuffled 5 |
| 237654 | pRK5_msfGFP-HMGB1-Shuffled 6 | For overexpression of msfGFP-HMGB1-Shuffled 6 |
| 237655 | pRK5_msfGFP-HMGB1-Shuffled 7 | For overexpression of msfGFP-HMGB1-Shuffled 7 |
| 237656 | pRK5_msfGFP-HMGB1-Shuffled 8 | For overexpression of msfGFP-HMGB1-Shuffled 8 |
| 237657 | pRK5_msfGFP-HMGB1-Shuffled 9 | For overexpression of msfGFP-HMGB1-Shuffled 9 |
| 237658 | pRK5_msfGFP-HMGB1-Shuffled 10 | For overexpression of msfGFP-HMGB1-Shuffled 10 |
| 237659 | pRK5_msfGFP-HMGB1-KS-FtoY | For overexpression of msfGFP-HMGB1-KS-FtoY |
| 237660 | pRK5_msfGFP-HMGB1-KS-FtoW | For overexpression of msfGFP-HMGB1-KS-FtoW |
| 237661 | PB_TetON_EGFP-NPM1 | For integration of EGFP-NPM1 |
| 237662 | PB_TetON_EGFP-NPM1-KS | For integration of EGFP-NPM1-KS |
| 237663 | PB_TetON_EGFP-NPM1-KS-FtoG | For integration of EGFP-NPM1-KS-FtoG |
| 237664 | PB_TetON_EGFP-NPM1-KS-FtoA | For integration of EGFP-NPM1-KS-FtoA |
| 237665 | PB_TetON_EGFP-NPM1-KS-0F | For integration of EGFP-NPM1-KS-0F |
| 237666 | pRK5_msfGFP-HMGB1-TDP43HP | For overexpression of msfGFP-HMGB1-TDP43HP |
| 237667 | pRK5_mEGFP-NPM1 | For overexpression of mEGFP-NPM1 |
| 237668 | pRK5_mEGFP-NPM1-KS | For overexpression of mEGFP-NPM1-KS |
| 237669 | pRK5_mEGFP-NPM1-TDP43HP | For overexpression of mEGFP-NPM1-TDP43HP |
| 237670 | pET22b-msfGFP-NPM1 | For expression of msfGFP-NPM1 in Ecoli |
| 237671 | pRK5_mEGFP-EWS-FLI1-KS | For overexpression of mEGFP-EWS-FLI1-KS |
| 237672 | pRK5_mEGFP-CRTC1-MAML2-KS | For overexpression of mEGFP-CRTC1-MAML2-KS |
| 237673 | pRK5_mEGFP-NUP98-HOXA9-KS | For overexpression of mEGFP-NUP98-HOXA9-KS |
| 237674 | pRK5_mEGFP-YAP-MAML2 | For overexpression of mEGFP-YAP-MAML2 |
| 237675 | pRK5_mEGFP-YAP-MAMLD1 | For overexpression of mEGFP-YAP-MAMLD1 |
| 237676 | pRK5_mEGFP-YAP-MAMLD1-KS | For overexpression of mEGFP-YAP-MAMLD1-KS |
| 237677 | pRK5_mEGFP-TAZ-CAMTA1-KS | For overexpression of mEGFP-TAZ-CAMTA1-KS |
| 237678 | pRK5_mEGFP-SS18-SSX1 | For overexpression of mEGFP-SS18-SSX1 |
| 237679 | pRK5_mEGFP-SS18-SSX1-KS | For overexpression of mEGFP-SS18-SSX1-KS |
| 237680 | pRK5_mEGFP-SS18-SSX2-KS | For overexpression of mEGFP-SS18-SSX2-KS |
| 237681 | pRK5_mEGFP-NONO-TFE3-KS | For overexpression of mEGFP-NONO-TFE3-KS |
| 237682 | pUC19_msfGFP-EWSR1-repair-tempate | For knock-in msfGFP to EWSR1 locus |
| 237683 | pX458_mCherry-EWSR1-gRNA | Cas9 from S. pyogenes with 2A-mCherry, gRNA to knock-in msfGFP to EWSR1 locus |
| 237684 | pUC19_msfGFP-FUS-repair-tempate | For knock-in msfGFP to FUS locus |
| 237685 | pX458_mCherry-FUS-gRNA | Cas9 from S. pyogenes with 2A-mCherry, gRNA to knock-in msfGFP to FUS locus |
| 237686 | p52K-GFP-linker-KS | For overexpression of 52K-GFP-linker-KS |
| 237687 | p52K-GFP-linker-KS-FtoA | For overexpression of 52K-GFP-linker-KS-FtoA |
| 237688 | p52K-KS | For overexpression of 52K-KS |
| 237689 | p52K-KS-FtoA | For overexpression of 52K-KS-FtoA |
| 237690 | p52K-GFP-KS | For overexpression of 52K-GFP-KS |
| 237691 | p52K-GFP-KS-FtoA | For overexpression of 52K-GFP-KS-FtoA |
| 237692 | p52K-linkerKS | For overexpression of 52K-linkerKS |
| 237693 | p52K-linkerKS-FtoA | For overexpression of 52K-linkerKS-FtoA |
| 238231 | pRK5_mCherry-P2A | For overexpression of mCherry control |
| 238232 | pRK5_mCherry_P2AT2A_GFP-nb | For overexpression of mCherry_P2AT2A_GFP-nb |
| 238233 | pRK5_mCherry_P2AT2A_GFP-nb-KS | For overexpression of mCherry_P2AT2A_GFP-nb-KS |
| 238234 | pRK5_mCherry_P2AT2A_GFP-nb-KS-FtoA | For overexpression of mCherry_P2AT2A_GFP-nb-KS-FtoA |
| 238235 | pRK5_mCherry_P2AT2A_GFP-nb-KS-FtoG | For overexpression of mCherry_P2AT2A_GFP-nb-KS-FtoG |
| 238236 | pRK5_mCherry_P2AT2A_GFP-nb-2xKS | For overexpression of mCherry_P2AT2A_GFP-nb-2xKS |
| 238237 | pRK5_mCherry_P2AT2A_GFP-nb-2xKS_FtoG | For overexpression of mCherry_P2AT2A_GFP-nb-2xKS_FtoG |
| 238238 | PB_TetON_mCherry-P2A | For integration of mCherry control |
| 238239 | PB_TetON_mCherry_P2AT2A_GFP-nb | For integration of mCherry_P2AT2A_GFP-nb |
| 238240 | PB_TetON_mCherry_P2AT2A_GFP-nb-KS | For integration of mCherry_P2AT2A_GFP-nb-KS |
| 238241 | PB_TetON_mCherry_P2AT2A_GFP-nb-KS-FtoA | For integration of mCherry_P2AT2A_GFP-nb-KS-FtoA |
| 238242 | PB_TetON_mCherry_P2AT2A_GFP-nb-KS-FtoG | For integration of mCherry_P2AT2A_GFP-nb-KS-FtoG |
| 238243 | PB_TetON_mCherry_P2AT2A_GFP-nb-2xKS | For integration of mCherry_P2AT2A_GFP-nb-2xKS |
| 238244 | PB_TetON_mCherry_P2AT2A_GFP-nb-2xKS_FtoG | For integration of mCherry_P2AT2A_GFP-nb-2xKS_FtoG |
| 238245 | pUC19_msfGFP-NPM1-repair-template for HCT116 | For knock-in msfGFP to NPM1 locus |
| 238246 | pUC19_msfGFP-NPM1-repair-template for U2OS | For knock-in msfGFP to NPM1 locus |
| 238247 | pUC19_msfGFP-SRRM2-repair-template | For knock-in msfGFP to SRRM2 locus |
| 238248 | pUC19_msfGFP-HP1a-repair-template | For knock-in msfGFP to HP1a locus |
| 238249 | pX458_mCherry-NPM1-gRNA for HCT116 | Cas9 from S. pyogenes with 2A-mCherry, gRNA to knock-in msfGFP to NPM1 locus |
| 238250 | pX458_mCherry-NPM1-gRNA for U2OS | Cas9 from S. pyogenes with 2A-mCherry, gRNA to knock-in msfGFP to NPM1 locus |
| 238251 | pX458_mCherry-SRRM2-gRNA | Cas9 from S. pyogenes with 2A-mCherry, gRNA to knock-in msfGFP to SRRM2 locus |
| 238252 | pRK5_mCherry-SURF6 | For overexpression of mCherry-SURF6 |
| 238253 | pRK5_mCherry-RPL18 | For overexpression of mCherry-RPL18 |
| 238254 | pRK5_EGFP-BRD4-NUT | For overexpression of EGFP-BRD4-NUT |
| 238255 | pRK5_EGFP-BRD4-NUT-KS | For overexpression of EGFP-BRD4-NUT-KS |
| 238256 | pRK5_EGFP-BRD4-NUT-KS-FtoG | For overexpression of EGFP-BRD4-NUT-KS-FtoG |
| 238257 | pRK5_EGFP-control | For overexpression of EGFP-control |
| 238258 | pRK5_mCherry-P300 | For overexpression of mCherry-P300 |
| 238259 | pRK5_mCherry-GFP-nb | For overexpression of mCherry-GFP-nb |
| 238260 | pRK5_mCherry-GFP-nb-KS | For overexpression of mCherry-GFP-nb-KS |
| 238261 | pRK5_ALFA-EGFP-NPM1 | For overexpression of ALFA-EGFP-NPM1 |
| 238262 | pRK5_ALFA-nb | For overexpression of ALFA-nb |
| 238263 | pRK5_ALFA-nb-KS | For overexpression of ALFA-nb-KS |
| 238264 | pRK5_V5-EGFP-NPM1 | For overexpression of V5-EGFP-NPM1 |
| 238265 | pRK5_V5-nb-noKS | For overexpression of V5-nb-noKS |
| 238266 | pRK5_V5-nb-KS | For overexpression of V5-nb-KS |
| 238267 | pRK5_V5-nb-KS-FtoG | For overexpression of V5-nb-KS-FtoG |
| 238268 | pRK5_VHH05-EGFP-NPM1 | For overexpression of VHH05-EGFP-NPM1 |
| 238269 | pRK5_VHH05-nb-noKS | For overexpression of VHH05-nb-noKS |
| 238270 | pRK5_VHH05-nb-KS | For overexpression of VHH05-nb-KS |
| 238271 | pRK5_VHH05-nb-KS-FtoG | For overexpression of VHH05-nb-KS-FtoG |
| 238272 | pRK5_TagBFP-P2A | For overexpression of TagBFP-control |
| 238273 | pRK5_TagBFP_P2AT2A_GFP-nb | For overexpression of TagBFP_P2AT2A_GFP-nb |
| 238274 | pRK5_TagBFP_P2AT2A_GFP-nb-KS | For overexpression of TagBFP_P2AT2A_GFP-nb-KS |
| 238275 | pRK5_TagBFP_P2AT2A_GFP-nb-KS-FtoG | For overexpression of TagBFP_P2AT2A_GFP-nb-KS-FtoG |
| 238276 | pRK5_mEGFP-YAP-MAML2-KS | For overexpression of mEGFP-YAP-MAML2-KS |
| 238277 | CFP-LacI | For overexpression of CFP-LacI |
| 238281 | pRK5_mCherry-P2AT2A-EGFP-BRD4-NUT | For overexpression of mCherry-P2AT2A-EGFP-BRD4-NUT |
| 238282 | pRK5_mCherry-P2AT2A-EGFP-BRD4-NUT-KS | For overexpression of mCherry-P2AT2A-EGFP-BRD4-NUT-KS |
| 238283 | pRK5_mCherry-P2AT2A-EGFP-BRD4-NUT-KS-FtoG | For overexpression of mCherry-P2AT2A-EGFP-BRD4-NUT-KS-FtoG |
| 238284 | pRK5_mCherry-P2AT2A-GFP-NUP98-DDX10 | For overexpression of mCherry-P2AT2A-GFP-NUP98-DDX10 |
| 238285 | pRK5_mCherry-P2AT2A-GFP-NUP98-DDX10-KS | For overexpression of mCherry-P2AT2A-GFP-NUP98-DDX10-KS |
| 238286 | pRK5_mCherry-P2AT2A-GFP-NUP98-DDX10-KS-FtoG | For overexpression of mCherry-P2AT2A-GFP-NUP98-DDX10-KS-FtoG |
| 238288 | TRE3G-mCherry-P2A-T2A-nanobody-KS-FtoA | For integration of mCherry-P2A-T2A-nanobody-KS-FtoA |
| 238289 | mscv_EGFP-IRES-NUP98-KDM5A | For overexpression of EGFP-IRES-NUP98-KDM5A |
| 238290 | mscv_EGFP-IRES-NUP98-KDM5A-KS | For overexpression of EGFP-IRES-NUP98-KDM5A-KS |
| 238291 | mscv_EGFP-IRES-NUP98-KDM5A-KS-FtoA | For overexpression of EGFP-IRES-NUP98-KDM5A-KS-FtoA |
| 238292 | mscv_EGFP-IRES-NUP98-KDM5A-KS-FtoG | For overexpression of EGFP-IRES-NUP98-KDM5A-KS-FtoG |
| 238293 | mscv_EGFP-NUP98-KDM5A | For overexpression of EGFP-NUP98-KDM5A |
| 238294 | mscv_EGFP-NUP98-KDM5A-KS | For overexpression of EGFP-NUP98-KDM5A-KS |
| 238295 | mscv_EGFP-NUP98-KDM5A-KS-FtoA | For overexpression of EGFP-NUP98-KDM5A-KS-FtoA |
| 238296 | Nanog-mNG | For RNA injection of Nanog-mNG into Zebrafish |
| 238297 | Nanog-mNG-KS-FtoG | For RNA injection of Nanog-mNG-KS-FtoG into Zebrafish |
| 238298 | Nanog-mNG-KS | For RNA injection of Nanog-mNG-KS into Zebrafish |