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Mechanism of MCUB-Dependent Inhibition of Mitochondrial Calcium Uptake.

Rai NK, Eberhardt DR, Balynas AM, MacEwen MJS, Bratt AR, Sancak Y, Chaudhuri D
J Cell Physiol. 2025 Apr;240(4):e70033. doi: 10.1002/jcp.70033. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
236786MCUabaa-Flag-PLYS1MCU-MCUB fusion in abaa pattern. Only the initial MCU has a mitochondrial targeting sequence.
236787MCUabbb-Flag-PLYS1MCU-MCUB fusion in abbb pattern. Only the initial MCU has a mitochondrial targeting sequence.
236788MCUabab-Flag-pLYS1MCU-MCUB fusion in abab pattern. Only the initial MCU has a mitochondrial targeting sequence.
236789MCUb-mVenus-pLJMMCUB tagged with mVenus for FRET / MW change for stoichiometry
236790MCUb-HA-pLYS5Clone MCUB into pLYS5 (Hygro resistance) with HA tag

Antibodies from Article