Viral delivery of an RNA-guided genome editor for transgene-free germline editing in Arabidopsis.
Weiss T, Kamalu M, Shi H, Li Z, Amerasekera J, Zhong Z, Adler BA, Song MM, Vohra K, Wirnowski G, Chitkara S, Ambrose C, Steinmetz N, Sridharan A, Sahagun D, Banfield JF, Doudna JA, Jacobsen SE
Nat Plants. 2025 Apr 22. doi: 10.1038/s41477-025-01989-9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 236570 | pMK003 | Cloning vector for PaqCI Golden Gate assembly of ISDra2 guides into ISDra2 plant expression vector |
| 236572 | pMK025 | Cloning vector for PaqCI Golden Gate assembly of ISYmu1 guides into ISYmu1 plant expression vector |
| 236573 | pMK435 | Cloning vector for PaqCI Golden Gate assembly of ISYmu1 gRNAs into TRV2 Architecture_B |
| 236574 | pTW2065 | ISYmu1 PDS3 gRNA2 TRV2 Architecture_A |
| 236575 | pTW2066 | ISYmu1 PDS3 gRNA2 TRV2 Architecture_B |
| 236576 | pTW2082 | ISYmu1 PDS3 gRNA12 TRV2 Architecture_B |
| 236577 | pTW2153 | ISYmu1 CHLl1 gRNA3 TRV2 Architecture_B |
| 236578 | pTW2154 | ISYmu1 CHLl1 gRNA4 TRV2 Architecture_B |
| 236579 | pTW2155 | ISYmu1 CHLl1 gRNA5 TRV2 Architecture_B |
| 236580 | pTW2156 | ISYmu1 CHLl1 gRNA6 TRV2 Architecture_B |
| 236581 | pTW2157 | ISYmu1 CHLl1 gRNA7 TRV2 Architecture_B |
| 236582 | pTW2159 | ISYmu1 CHLl1 gRNA9 TRV2 Architecture_B |
| 236583 | pTW1169 | TRV1 |