Pablo Rivera-Fuentes Lab: Rivera-Fuentes plasmids March 2025
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 236545 | NoLS-Grx1-roGFP2 | Expressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells with localization in the nucleoli. |
| 236546 | Grx1-roGFP2-Coilin | Expressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells with localization in Cajal bodies. |
| 236547 | Grx1-roGFP2-Dcp1b | Expressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells with localization in processing bodies. |
| 236548 | G3BP1-Grx1-roGFP2 | Expressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells with localization in stress granules. |
| 236550 | H2B-Grx1-roGFP2 | Expressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells with localization in the nucleus. |
| 236551 | NES-Grx1-roGFP2 | Expressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells with localization in the cytoplasm. |
| 236552 | DONOR plasmid | Contains the insert sequence IRES-HaloTag-T2A-PuroR flanked by microhomologies for incorporation downstream of HSPA5 (BiP/GRP78). |
| 236553 | CRISPR plasmid | Encodes Cas9 and two guideRNAs, one to cut the genome downstream of HSPA5 (BiP/GRP78) and one to linearize the DONOR plasmid. |