Optimized vectors for genetic engineering of Aureobasidium pullulans.

Colarusso A, Williams A, Gladfelter AS, Wirshing ACE, Lew DJ
bioRxiv [Preprint]. 2025 Jan 27:2025.01.25.634885. doi: 10.1101/2025.01.25.634885. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
236472	pAP-U2-1	Plasmid for integration at the native URA3 locus with 3xmCherry and 3xGFP under control of the strong bidirectional SpH2A/B promoter.
236473	pAP-U2-2	Plasmid for integration at the native URA3 locus with 3xmCherry under control of the SpH2B promoter and 3xGFP under control of the ScACT1 promoter.
236474	pAP-U2-3	Plasmid for integration at the native URA3 locus with 3xmCherry under control of the ScACT1 promoter and 3xGFP under control of the ApACT1 promoter.
236475	pAP-U2-4	Plasmid for integration at the native URA3 locus with 3xmCherry under control of the ScACT1 promoter and 3xGFP under control of the ApTUB1 promoter.
236476	pAPInt-GFP-Nat	Plasmid for deleting or C-terminally tagging endogenous genes with GFP and selection on Nat.
236477	pAPInt-GFP-Hyg	Plasmid for deleting or C-terminally tagging endogenous genes with GFP and selection on Hyg.
236478	pAPInt-GFP-G418	Plasmid for deleting or C-terminally tagging endogenous genes with GFP and selection on G418.
236479	pAPInt-sfGFP-Nat	Plasmid for deleting or C-terminally tagging endogenous genes with sfGFP and selection on Nat.
236480	pAPInt-sfGFP-Hyg	Plasmid for deleting or C-terminally tagging endogenous genes with sfGFP and selection on Hyg.
236481	pAPInt-sfGFP-G418	Plasmid for deleting or C-terminally tagging endogenous genes with sfGFP and selection on G418.
236482	pAPInt-mNeonGreen-Nat	Plasmid for deleting or C-terminally tagging endogenous genes with mNeonGreen and selection on Nat.
236483	pAPInt-mNeonGreen-Hyg	Plasmid for deleting or C-terminally tagging endogenous genes with mNeonGreen and selection on Hyg.
236485	pAPInt-mStayGold-Nat	Plasmid for deleting or C-terminally tagging endogenous genes with mStayGold and selection on Nat.
236486	pAPInt-mStayGold-Hyg	Plasmid for deleting or C-terminally tagging endogenous genes with mStayGold and selection on Hyg.
236487	pAPInt-mStayGold-G418	Plasmid for deleting or C-terminally tagging endogenous genes with mStayGold and selection on G418.
236488	pAPInt-mCherry-Nat	Plasmid for deleting or C-terminally tagging endogenous genes with mCherry and selection on Nat.
236489	pAPInt-mCherry-Hyg	Plasmid for deleting or C-terminally tagging endogenous genes with mCherry and selection on Hyg.
236490	pAPInt-mCherry-G418	Plasmid for deleting or C-terminally tagging endogenous genes with mCherry and selection on G418.
236491	pAPInt-mScarlet-Nat	Plasmid for deleting or C-terminally tagging endogenous genes with mScarlet and selection on Nat.
236492	pAPInt-mScarlet-Hyg	Plasmid for deleting or C-terminally tagging endogenous genes with mScarlet and selection on Hyg.
236493	pAPInt-mScarlet-G418	Plasmid for deleting or C-terminally tagging endogenous genes with mScarlet and selection on G418.
236494	pAPInt-Dendra2-Nat	Plasmid for deleting or C-terminally tagging endogenous genes with Dendra2 and selection on Nat.
236495	pAPInt-Dendra2-Hyg	Plasmid for deleting or C-terminally tagging endogenous genes with Dendra2 and selection on Hyg.
236496	pAPInt-Dendra2-G418	Plasmid for deleting or C-terminally tagging endogenous genes with Dendra2 and selection on G418.