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A dual-purification system to isolate mitochondrial subpopulations.

Cunningham CN, Van Vranken JG, Larios J, Heyden K, Gygi SP, Rutter J
J Cell Sci. 2025 Mar 13:jcs.263693. doi: 10.1242/jcs.263693. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
236400pQCXIP-C1(1-29)-EGFP-APEX2-V5Expresses a fluorescent Endoplasmic Reticulum-localized APEX2
236401pQCXIP-Pex3-EGFP-APEX2-V5Expresses a fluorescent peroxisome-localized APEX2
236402pQCXIP-LAMP1-EGFP-APEX2-V5Expresses a fluorescent lysosome-localized APEX2
236403pQCXIP-EGFP-APEX2-V5-EEA1Expresses a fluorescent endosome-localized APEX2
236404pQCXIP-EGFP-APEX2-V5-GiantinExpresses a fluorescent Golgi-localized APEX2
236405pQCXIP-EGFP-ADRP-APEX2-V5Expresses a fluorescent lipid droplet-localized APEX2

Antibodies from Article