Radhakrishnan Mahadevan Lab: Quorum Sensing-Driven CRISPR/dCas12a Circuit for Dynamic Regulation of Metabolic Pathways
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 236037 | pQdCas12a.luxR(mut)-empty | The plasmid pQdCas12a.luxR(mut)-empty expresses the dCas12a endonuclease. Additionally, it contains a mutation in the luxR gene. |
| 236038 | pQdCas12a.sggfp(A) | The plasmid pQdCas12a.sggfp(A) expresses the dCas12a endonuclease and the sgRNA (design A) targeting the gfp gene. |
| 236039 | pQdCas12a.luxR(mut)-sggfp(A) | The plasmid pQdCas12a.luxR(mut)-sggfp(A) expresses the dCas12a endonuclease and the sgRNA (design A) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene. |
| 236040 | pQdCas12a.sggfp(B) | The plasmid pQdCas12a.sggfp(B) expresses the dCas12a endonuclease and the sgRNA (design B) targeting the gfp gene. |
| 236041 | pQdCas12a.luxR(mut)-sggfp(B) | The plasmid pQdCas12a.luxR(mut)-sggfp(B) expresses the dCas12a endonuclease and the sgRNA (design B) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene. |
| 236042 | pQdCas12a.sggfp(C) | The plasmid pQdCas12a.sggfp(C) expresses the dCas12a endonuclease and the sgRNA (design C) targeting the gfp gene. |