Ultra-Efficient Integration of Gene Libraries onto Yeast Cytosolic Plasmids.
Pisera A, Yu Y, Williams RL, Liu CC
bioRxiv [Preprint]. 2024 Nov 30:2024.11.29.626108. doi: 10.1101/2024.11.29.626108.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 235771 | pYY10-2µ-TP901 | 2µ plasmid for constitutive expression of TP901 under RNR2 promoter. |
| 235772 | pYY13-URA3-TP901LP | Plamids for generation of donor DNA for construction of a TP901-compatible landing pad p1 with URA3 selection marker. |
| 235773 | pYY62-P1int-TP901-GFPdropout | Plasmid used for constructing donor DNA for TP901-compatible p1 integration. This plasmid is a sfGFP dropout plasmid where sfGFP can be replaced by GOIs using Golden Gate Assembly. |
| 235976 | pRW404-2µ-BXB1 | 2µ plasmid for constitutive expression of BXB1 under RNR2 promoter. |
| 235977 | pRW398-URA3-BXB1-LP | Plamids for generation of donor DNA for construction of a BXB1-compatible landing pad p1 with URA3 selection marker. |
| 235979 | pRW402-P1int-BXB1 | Plasmid used for constructing donor DNA for BXB1-compatible p1 integration. You can insert your GOIs into this plasmid by using Golden Gate Assembly. |