Directed evolution of a sequence-specific covalent protein tag for RNA labeling.
Huang R, Ting AY
Proc Natl Acad Sci U S A. 2025 Mar 4;122(9):e2422085122. doi: 10.1073/pnas.2422085122. Epub 2025 Feb 26.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 235187 | pCTcon2-dHUH | To display dHUH on yeast surface |
| 235188 | pCTcon2-rHUH(G5) | To display rHUH(G5) on yeast surface |
| 235189 | pCTcon2-rHUH_Y96F(G5) | To display catalytic inactivated rHUH(G5) with Y96F mutation on yeast surface |
| 235190 | pCTcon2-rHUH | To display rHUH on yeast surface |
| 235191 | pCTcon2-rHUH_Y96F | To display catalytic inactivated rHUH with Y96F mutation on yeast surface |
| 235192 | pTetON-rHUH | To express rHUH protein in mammalian cells |
| 235194 | pTD68-rHUH | To express rHUH-myc in bacteria |
| 235195 | pTD68-rHUH_Y96F | To express catalytic inactivated rHUH-myc protein with Y96F mutation in bacteria |