Photosynthetic demands on translational machinery drive retention of redundant tRNA metabolism in plant organelles.
DeTar RA, Chustecki JM, Martinez-Hottovy A, Ceriotti LF, Broz AK, Lou X, Sanchez-Puerta MV, Elowsky C, Christensen AC, Sloan DB
Proc Natl Acad Sci U S A. 2024 Dec 24;121(52):e2421485121. doi: 10.1073/pnas.2421485121. Epub 2024 Dec 18.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 202646 | Shim_CytC_TP_GFP_pK7FWG2 | Determine where transit peptide targets GFP in N. benthamiana |
| 202647 | Shim_AspRS_TP_GFP_pK7FWG2 | Determine where transit peptide targets GFP in N. benthamiana |
| 202648 | Shim_GlyRS_TP_GFP_pK7FWG2 | Determine where transit peptide targets GFP in N. benthamiana |
| 202649 | Shim_AsnRS_TP_GFP_pK7FWG2 | Determine where transit peptide targets GFP in N. benthamiana |
| 202650 | Shim_MetRS_TP_GFP_pK7FWG2 | Determine where transit peptide targets GFP in N. benthamiana |
| 202651 | Shim_ProRS_TP_GFP_pK7FWG2 | Determine where transit peptide targets GFP in N. benthamiana |
| 202652 | Shim_ThrRS_TP_GFP_pK7FWG2 | Determine where transit peptide targets GFP in N. benthamiana |
| 202653 | Shim_TrpRS_TP_GFP_pK7FWG2 | Determine where transit peptide targets GFP in N. benthamiana |
| 228370 | Athal_PheRS_TP_pK7fWG2_IVD_FP611 | Expresses N-term transit peptide from Arabidopsis PheRS At3g58140 with demonstrated dual mito/plastid targeting; proof-of-concept for Plasmid 228371 |
| 228371 | empty_pK7fWG2_IVD_FP611 | Empty dual expression vector for determining subcellular localization for protein of interest |
| 228372 | Scon_PheRS_TP_pK7fWG2_IVD_FP611 | Expresses N-term transit peptide from Silene conica PheRS (Warren et al., 2023) with demonstrated plastid targeting; proof-of-concept for Plasmid 228371 |
| 228373 | Shim_AsnRS_419.110_SHI02794_TP_GFP_pK7fWG2_IVD_FP611 | Expresses N-term transit peptide from Sapria himalayana Asparagine aminoacyl-tRNA synthetase evm.model.fragScaff scaffold 419.110 (SHI02794); determination of S. himalayana AsnRS target localization |
| 228374 | Shim_AspRS_3212.91_TP_GFP_pK7fWG2_IVD_FP611 | Expresses N-term transit peptide from Sapria himalayana Aspartate aminoacyl-tRNA synthetase evm.model.fragScaff scaffold 3212.91; determination of S. himalayana AspRS target localization |
| 228375 | Shim_CytC_87.18_SHI04112_TP_GFP_pK7fWG2_IVD_FP611 | Expresses N-term transit peptide from Sapria himalayana Cytochrome C with demonstrated mito targeting; proof-of-concept for Plasmid 228371 |
| 228376 | Shim_GluRS_87.31.3_TP_GFP_pK7fWG2_IVD_FP611 | Expresses N-term transit peptide from Sapria himalayana Glutamate aminoacyl-tRNA synthetase evm.model.fragScaff_scaffold_87.31.3; determination of S. himalayana GluRS target localization |
| 228377 | Shim_GlyRS_87.19_SHI04113_TP_GFP_pK7fWG2_IVD_FP611 | Expresses N-term transit peptide from S. himalayana Glycine aminoacyl-tRNA synthetase evm.model.fragScaff scaffold 87.19 (SHI04113RA); determination of Sapria himalayana GlyRS target localization |
| 228378 | Shim_MetRS_440.109_TP_GFP_pK7fWG2_IVD_FP611 | Expresses N-term transit peptide from Sapria himalayana Methionine aminoacyl-tRNA synthetase evm.model.fragScaff scaffold 440.10; determination of S. himalayana MetRS target localization |
| 228379 | Shim_ProRS_3278.3.7_SHI28108_TP_GFP_pK7fWG2_IVD_FP611 | Expresses N-term transit peptide from Sapria himalayana Proline aminoacyl-tRNA synthetase evm.model.fragScaff scaffold 3278.3.7 (SHI28108RA); determination of S. himalayana ProRS target localization |
| 228380 | Shim_ThrRS_3895.3_TP_GFP_pK7fWG2_IVD_FP611 | Expresses N-term transit peptide from Sapria himalayana Threonine aminoacyl-tRNA synthetase evm.model.fragScaff scaffold 3895.3; determination of S. himalayana ThrRS target localization |
| 228381 | Shim_TrpRS_2406.7.1_SHI16564_TP_GFP_pK7fWG2_IVD_FP611 | Expresses N-term transit peptide from Sapria himalayana Tryptophan aminoacyl-tRNA synthetase (evm.model.fragScaff_scaffold_2406.7.1/SHI16564RA); to determine S. himalayana TrpRS target localization |