Validation of heat-inducible Ixodes scapularis HSP70 and tick-specific 3xP3 promoters in ISE6 cells.
Pham M, Hoffmann HH, Kurtti TJ, Chana R, Garcia-Cruz O, Aliabadi S, Gulia-Nuss M
iScience. 2024 Jul 8;27(8):110468. doi: 10.1016/j.isci.2024.110468. eCollection 2024 Aug 16.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 220091 | Is3xP3-mCherry | Expresses in ISE6 (Ixodes scapularis) cells, heat inducible. Promoter consists of 3 Pax 6 sites from 3xP3 with a minimal IsHSP70 promoter. Plasmid backbone derived from pGL-4.79 [Rluc] (Promega) |
| 220092 | IsHSP70-mCherry | Expresses in ISE6 (Ixodes scapularis) cells, heat inducible. Plasmid backbone derived from pGL-4.79 [Rluc] (Promega) |
| 220137 | pGL 4.79-mCherry | promoterless vector |