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Structure and organization of full-length Epidermal Growth Factor Receptor in extracellular vesicles by cryo-electron tomography.

Gonzalez-Magaldi M, Gullapalli A, Papoulas O, Liu C, Leung AY, Guo L, Brilot A, Marcotte EM, Ke Z, Leahy DJ
bioRxiv [Preprint]. 2024 Nov 28:2024.11.25.625301. doi: 10.1101/2024.11.25.625301. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
234988pHA StreptagII 3C NIS EABRFor production of Extracellular Vesicles (EVs), with a Strep-tag II labeled Sodium-iodide symporter (NIS), and an HA epitope at its N-terminus on their surface
234990pNA 3C myc scfvFLAGExpression of transmembrane region of Neuraminidase protein fused to an anti FLAG scFv antibody fragment for displaying on Viral like particles (VLPs) when cotranfected with GAG protein
234991pNA 3C myc scfvHAExpression of transmembrane region of Neuraminidase protein fused to an anti HA scFv antibody fragment for displaying on Viral like particles (VLPs) when cotranfected with GAG proteins
234992pBS CMV GAGFor production of Viral like particles (VLPs) by expressing the MLV GAG protein in mammalian cells

Antibodies from Article