Structure and organization of full-length Epidermal Growth Factor Receptor in extracellular vesicles by cryo-electron tomography.
Gonzalez-Magaldi M, Gullapalli A, Papoulas O, Liu C, Leung AY, Guo L, Brilot A, Marcotte EM, Ke Z, Leahy DJ
bioRxiv [Preprint]. 2024 Nov 28:2024.11.25.625301. doi: 10.1101/2024.11.25.625301.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 234987 | pEABR NA 3C FLAG SA | For production of Extracellular Vesicles (EVs), with the transmembrane region of Neuraminidase protein fused to Streptavidin on the vesicles surface |
| 234988 | pHA StreptagII 3C NIS EABR | For production of Extracellular Vesicles (EVs), with a Strep-tag II labeled Sodium-iodide symporter (NIS), and an HA epitope at its N-terminus on their surface |
| 234989 | pHA TST 3C SMO EABR | For production of Extracellular Vesicles (EVs), with the receptor Smoothened (Smo), Twin-Strep-tag (TST), and an HA tag at its N-terminus on their surface |
| 234990 | pNA 3C myc scfvFLAG | Expression of transmembrane region of Neuraminidase protein fused to an anti FLAG scFv antibody fragment for displaying on Viral like particles (VLPs) when cotranfected with GAG protein |
| 234991 | pNA 3C myc scfvHA | Expression of transmembrane region of Neuraminidase protein fused to an anti HA scFv antibody fragment for displaying on Viral like particles (VLPs) when cotranfected with GAG proteins |
| 234992 | pBS CMV GAG | For production of Viral like particles (VLPs) by expressing the MLV GAG protein in mammalian cells |
| 234993 | pBS CMV NA 3C FLAG SA | Expression of transmembrane region of Neuraminidase protein fused to streptavidin protein for displaying on viral like particles (VLPs) when cotranfected with GAG protein |
| 234994 | pStreptagII 3C EGFR EABR | For production of Extracellular Vesicles (EVs), with Epithelial Growth Factor Receptor on their surface, Strep-tag II labeled on its N-terminus |
| 234995 | pStreptagII 3C SMO | Expression of a Strep-tag II labeled on its N-terminus receptor, with the Smoothened (Smo) receptor |
| 234996 | pStreptagIIx2 Alfatag 3C InsR EABR | For production of Extracellular Vesicles (EVs), with the Insulin Receptor Strep-tag II labeled on their surface |