Engineering mtDNA Deletions by Reconstituting End-Joining in Human Mitochondria.
Fu Y, Land M, Cui R, Kavlashvili T, Kim M, Lieber T, Ryu KW, DeBitetto E, Masilionis I, Saha R, Takizawa M, Baker D, Tigano M, Reznik E, Sharma R, Chaligne R, Thompson CB, Pe'er D, Sfeir A
bioRxiv [Preprint]. 2024 Oct 17:2024.10.15.618543. doi: 10.1101/2024.10.15.618543.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 234948 | TLCV2-ApaLI-T2A-EGFP-Puro | Doxycycline-inducible mitochondria-localized ApaLI expressing plasmid |
| 234949 | TLCV2-Scal-T2A-EGFP-Puro | Doxycycline-inducible mitochondria-localized ScaI expressing plasmid |
| 234950 | pHAGE2-EF1a-E1MLS-2xMyc-Ku-IRES-Hygro | Human codon optimized Ku (Mycobacterium) with N-terminal E1 MLS expressing plasmid |
| 234951 | pHAGE2-EF1a-E1MLS-2xFLAG-LigD-IRES-tdTomato | Human codon optimized LigD (Mycobacterium) with N-terminal E1 MLS expressing plasmid |
| 234952 | pHAGE2-EF1a-E1MLS-2×FLAG-T4Lig-IRES-tdTomato | Human codon optimized T4 DNA Ligase with N-terminal E1 MLS expressing plasmid |
| 234953 | pHAGE2-EF1a-E1MLS-2xFLAG-T4Lig-IRES-Neo | Human codon optimized T4 DNA Ligase with N-terminal E1 MLS expressing plasmid |
| 234954 | pHAGE2-EF1a-FOATPMLS-2xFLAG-T4Lig-IRES-Neo | Human codon optimized T4 DNA Ligase with N-terminal F0ATP MLS expressing plasmid |