Delivering progranulin to neuronal lysosomes protects against excitotoxicity.
Davis SE, Roth JR, Aljabi Q, Hakim AR, Savell KE, Day JJ, Arrant AE
J Biol Chem. 2021 Sep;297(3):100993. doi: 10.1016/j.jbc.2021.100993. Epub 2021 Jul 21.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 234872 | PGK-HA-PGRN | Lentiviral plasmid expressing HA-tagged human progranulin. |
| 234873 | PGK-HA-PGRN-LAMP1TM | Lentiviral plasmid expressing HA-tagged human progranulin that is fused to the transmembrane domain and cytosolic tail of LAMP-1. Enables lysosomal delivery of progranulin without secretion. |
| 234874 | PGK-GFP-LAMP1TM | Lentiviral plasmid expressing GFP fused to the transmembrane domain and cytosolic tail of LAMP-1. Designed as a control vector for PGK-HA-PGRN-LAMP1TM. |
| 234875 | hSyn-IRES2-GFP | Lentiviral plasmid with a multiple cloning site followed by IRES2-GFP. |
| 234876 | hSyn-HA-PGRN-IRES2-GFP | Lentiviral plasmid expressing HA-tagged human progranulin followed by IRES2-GFP. |
| 234877 | hSyn-HA-PGRN-LAMP1TM-IRES-GFP | Lentiviral plasmid expressing HA-tagged human progranulin that is fused to the transmembrane domain and cytosolic tail of LAMP-1. Enables lysosomal delivery of progranulin without secretion. |