Reprogramming site-specific retrotransposon activity to new DNA sites.
Fell CW, Villiger L, Lim J, Hiraizumi M, Tagliaferri D, Yarnall MTN, Lee A, Jiang K, Kayabolen A, Krajeski RN, Schmitt-Ulms C, Ramani H, Yousef SM, Roberts N, Vakulskas CA, Nishimasu H, Abudayyeh OO, Gootenberg JS
Nature. 2025 Apr 9. doi: 10.1038/s41586-025-08877-4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 234826 | pDY1330 STITCHR pCMV-nCas9-XTEN-v170_R2Tocc | STITCHR R2Tocc editor |
| 234827 | pDY1656 STITCHR Gluc AAVS1 payload | Gluc exon 2 with 50bp AAVS1 homology arms |
| 234829 | pDY1440 STITCHR GFP NOLC1 payload | GFP cargo with 50bp NOLC homology arms |
| 234832 | pDY1513 AAVS1 sgRNA | sgRNA for AAVS1 STITCHR insertion |
| 234834 | pDY1623 NOLC1 sgRNA 2 | sgRNA 2 for NOLC1 STITCHR insertion |