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Efficient in vivo labeling of endogenous proteins with SMART delineates retina cellular and synaptic organization

Zhao C, Cao Y, Ibrahim N, Wang Y, Martemyanov KA
Nature Communications 2025 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
234724pCAG-eCas9-T2A-EGFP-U6-gRNA-no ITR and f1 oriAll-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9 expression in neurons. The plasmid lacks AAV2 ITR and f1 ori elements, enabling more efficient transfection and expression.
234737pCAG-eCas9-T2A-EGFP-U6-gRNA targeting Nrl-no ITR and f1 oriAll-in-one CRISPR/Cas9 vector encodes high-fidelity eSpCas9 and a gRNA targeting Nrl, efficiently reprogramming rod precursors into cone-like cells in the mouse retina.

Antibodies from Article