Esteban Serra Lab: Serra Lab Plasmids

Unpublished

Plasmids from Article

ID	Plasmid	Purpose
234714	pTcTurboID-GW	Used to express any CDS fused to 3xHA-TurboID (N-terminal) in T. cruzi. Tetracycline regulated. Modified from Alonso et al. (2014).
234715	pTcTurboID-GW_GFP	Used to express GFP fused to 3xHA-TurboID (N-terminal) in T. cruzi. Tetracycline regulated. Used as proximity labelling cytoplasmatic spatial control. CDS can be cloned in frame to GFP using EcoRV.
234716	pTcTurboID-GW_GFP-NLS	Used to express GFP-3xNLS fused to 3xHA-TurboID (N-terminal) in T. cruzi. Tetracycline regulated. Used as proximity labelling nuclear spatial control.