A suite of pre-assembled, pET28b-based Golden Gate vectors for efficient protein engineering and expression.
Gaur D, Wohlever ML
Protein Sci. 2025 Apr;34(4):e70106. doi: 10.1002/pro.70106.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 234674 | p344_DG | Golden gate domesticated pET28b with His6-3C |
| 234675 | p345_DG | Golden gate domesticated pET28b with His10-3C |
| 234676 | p346_DG | Golden gate domesticated pET28b with His10-TEV |
| 234677 | p347_DG | Golden gate domesticated pET28b with His10-Thrombin |
| 234678 | p348_DG | Golden gate domesticated pET28b with His10-MBP-3C |
| 234679 | p349_DG | Golden gate domesticated pET28b with His10-GST-3C |
| 234680 | p350_DG | Golden gate domesticated pET28b with His10-bdNEDD8 |
| 234681 | p351_DG | Golden gate domesticated pET28b with His10-mNeongreen-3C |
| 234682 | p352_DG | Golden gate domesticated pET28b with His10-mNeongreen-bdNEDD8 |
| 234683 | p361_DG | Golden gate domesticated pET28b with His6-3C-3XFLAG-SUMO and Thrombin-HiBiT |
| 234685 | p363_DG | Golden gate domesticated pET28b with His10-bdNEDD8 and Sortase |
| 234686 | p364_DG | Golden gate domesticated pET28b with His10-bdNEDD8 and Opsin |
| 234689 | p367_DG | Golden gate domesticated pET28b with His10-bdNEDD8 and 2X Strep tag |
| 234690 | p324_DG | Golden gate domesticated pET28b with TEV-His6 |
| 234691 | p368_DG | Golden gate domesticated pET28b with 3C-His6 |
| 234692 | p369_DG | Golden gate domesticated pET28b with 3C-His10 |
| 234693 | p370_DG | Golden gate domesticated pET28b with 3C-mNeongreen-His10 |
| 234695 | p277_DG | Golden gate domesticated pET28b for scarless cloning with His6-3C |
| 234696 | p353_DG | Golden gate domesticated pET28b for scarless cloning with His10-3C |
| 234697 | p354_DG | Golden gate domesticated pET28b for scarless cloning with His10-TEV |
| 234698 | p355_DG | Golden gate domesticated pET28b for scarless cloning with His10-Thrombin |
| 234699 | p356_DG | Golden gate domesticated pET28b for scarless cloning with His10-MBP-3C |
| 234700 | p357_DG | Golden gate domesticated pET28b for scarless cloning with His10-GST-3C |
| 234701 | p358_DG | Golden gate domesticated pET28b for scarless cloning with His10-bdNEDD8 |
| 234702 | p359_DG | Golden gate domesticated pET28b for scarless cloning with His10-mNeongreen-3C |
| 234703 | p360_DG | Golden gate domesticated pET28b for scarless cloning with His10-mNeongreen-bdNEDD8 |