Metastable condensates suppress conversion to amyloid fibrils.
Das T, Zaidi F, Farag M, Ruff KM, Messing J, Taylor JP, Pappu RV, Mittag T
bioRxiv [Preprint]. 2024 Mar 3:2024.02.28.582569. doi: 10.1101/2024.02.28.582569.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 234617 | A1-LCD 5W D262V | Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) D262V with partial W mutant |
| 234619 | hnRNPA1-FLAG-IRES-eGFP | Mammalian expression of full-length hnRNPA1 with C-terminal FLAG tag co-expressing with eGFP via IRES sequence |
| 234620 | hnRNPA1_D262V-FLAG-IRES-eGFP | Mammalian expression of full-length hnRNPA1 D262V with C-terminal FLAG tag co-expressing with eGFP via IRES sequence |
| 234621 | hnRNPA1_allW_D262V-FLAG-IRES-eGFP | Mammalian expression of full-length hnRNPA1 D262V with all aromatic amino acids in the C-terminal LCD mutated to W keeping the hexapeptide fibril core (SYNDFG) and the PY-NLS intact. |