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Metastable condensates suppress conversion to amyloid fibrils.

Das T, Zaidi F, Farag M, Ruff KM, Messing J, Taylor JP, Pappu RV, Mittag T
bioRxiv [Preprint]. 2024 Mar 3:2024.02.28.582569. doi: 10.1101/2024.02.28.582569. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
234607A1-LCD D262VBacterial expression of N-terminally 6His tagged hnRNPA1 D262V LCD (186-320)
234608A1-LCD D262NBacterial expression of N-terminally 6His tagged hnRNPA1 D262N LCD (186-320)
234609A1-LCD allWBacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with all aromatic amino acids mutated to W keeping the hexapeptide fibril core (SYNDFG) intact.
234610A1-LCD -4D+4VBacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with 4 aspartates mutated to valine
234611A1-LCD -4D+4NBacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with 4 aspartates mutated to asparagine
234612A1-LCD -3G1S+4VBacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with 3 Gly and 1 Ser mutated to Asn
234613A1-LCD -3D+3VBacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with 3 aspartates mutated to valine
234614A1-LCD -3D+3NBacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with 3 aspartates mutated to asparagine
234615A1-LCD allW D262NBacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) D262N with all aromatic amino acids mutated to W keeping the hexapeptide fibril core (SYNDFG) intact.
234616A1-LCD allW D262VBacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) D262V with all aromatic amino acids mutated to W keeping the hexapeptide fibril core (SYNDFG) intact.
234617A1-LCD 5W D262VBacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) D262V with partial W mutant
234618A1-LCD 11W D262VBacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) D262V with partial W mutant
234619hnRNPA1-FLAG-IRES-eGFPMammalian expression of full-length hnRNPA1 with C-terminal FLAG tag co-expressing with eGFP via IRES sequence
234620hnRNPA1_D262V-FLAG-IRES-eGFPMammalian expression of full-length hnRNPA1 D262V with C-terminal FLAG tag co-expressing with eGFP via IRES sequence
234621hnRNPA1_allW_D262V-FLAG-IRES-eGFPMammalian expression of full-length hnRNPA1 D262V with all aromatic amino acids in the C-terminal LCD mutated to W keeping the hexapeptide fibril core (SYNDFG) and the PY-NLS intact.

Antibodies from Article