A cysteine-less and ultra-fast split intein rationally engineered from being aggregation-prone to highly efficient in protein trans-splicing.
Humberg C, Yilmaz Z, Fitzian K, Dorner W, Kummel D, Mootz HD
Nat Commun. 2025 Mar 19;16(1):2723. doi: 10.1038/s41467-025-57596-x.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 234486 | pET28b_H6-Smt3-AesC-GFP | Superfolder GFP C-terminally fused to His-Smt3-tagged AesC split intein |
| 234487 | pMAL-c2X_MBP-CLmN-H6 | Maltose binding protein N-terminally fused to His-tagged CLmN split intein |