Enhanced RNA-targeting CRISPR-Cas technology in zebrafish.
Moreno-Sanchez I, Hernandez-Huertas L, Nahon-Cano D, Martinez-Garcia PM, Treichel AJ, Gomez-Marin C, Tomas-Gallardo L, da Silva Pescador G, Kushawah G, Egidy R, Perera A, Diaz-Moscoso A, Cano-Ruiz A, Walker JA 2nd, Munoz MJ, Holden K, Galceran J, Nieto MA, Bazzini AA, Moreno-Mateos MA
Nat Commun. 2025 Mar 16;16(1):2591. doi: 10.1038/s41467-025-57792-9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 234479 | pT3TS-Hf-RfxCas13d-HA | Plasmid to carry out IVT of High-fidelity RfxCas13d (human codon-optimized) |
| 234480 | pET28b-Hf-RfxCas13d-His | Plasmid for bacterial expression and purification of High-fidelity RfxCas13d protein (human codon-optimized) |
| 234482 | pET28b-DiCas7-11-His | Plasmid for bacterial expression and purification of DiCas7-11 protein (human codon-optimized) |
| 234485 | pT3TS-RfxCas13d-hei | Plasmid to carry out IVT of RfxCas13d with myc tag and two oNLS (human codon-optimized) |