Localization of PPM1H phosphatase tunes Parkinson's disease-linked LRRK2 kinase-mediated Rab GTPase phosphorylation and ciliogenesis
Wondwossen M Yeshaw , Ayan Adhikari, Claire Y Chiang, Herschel S Dhekne, Paulina S Wawro, Suzanne R Pfeffer
Proc Natl Acad Sci U S A. 2023 Oct 31;120(44)
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 207333 | pET15b+His SUMO PPM1H | Bacterial expression of His SUMO PPM1H |
| 207334 | pET15b+His SUMO Δ37 PPM1H | Bacterial expression of His SUMO Δ37 PPM1H |
| 207335 | pCMV5D HA-Δ10 PPM1H | Mammalian expression of HA-Δ10 PPM1H |
| 207336 | pCMV5D HA-Δ20 PPM1H | Mammalian expression of HA-Δ20 PPM1H |
| 207337 | pCMV5D HA-Δ34 PPM1H | Mammalian expression of HA-Δ34 PPM1H |
| 207338 | pCMV5D HA-Δ37 PPM1H | Mammalian expression of HA-Δ37 PPM1H |
| 207339 | pCMV5D HA-Δ44 PPM1H | Mammalian expression of HA-Δ44 PPM1H |
| 207340 | pCMV5D HA-Δ71 PPM1H | Mammalian expression of HA-Δ71 PPM1H |
| 207341 | pCMV5D HA-L2D/V9D/I19D PPM1H | Mammalian expression of HA-L2D/V9D/I19D PPM1H |
| 207342 | pET15b+His SUMO Rab10(Q68L) | Bacterial expression of His SUMO Rab10(Q68L) |
| 207343 | pCMV5D HA-Δ115-133, 204-217 PPM1H | Mammalian expression of HA-Δ115-133, 204-217 PPM1H |
| 207344 | pCMV5D HA-Δ37, 115-133, 204-217 PPM1H | Mammalian expression of HA-Δ37, 115-133, 204-217 PPM1H |
| 207349 | pmcb306-GFP-Rab12-mito | Mammalian expression of Rab12-mito |
| 207350 | CSII-mApple | Mammalian expression of mApple |
| 207351 | CSII-PPM1H-mApple-mito | Mammalian expression of PPm1H-mito |
| 208366 | pcDNA 3.1 myc-Rab10 | Mammalian expression of myc-tagged human Rab10 |
| 208367 | pcDNA 3.1 myc-Rab10-mito | Mammalian expression of myc-tagged human Rab10 with C-terminal mito signal |
| 208368 | pCMV5D HA-PPM1H S124A | Mammalian expression of HA-tagged human PPM1H S124A |
| 208369 | pCMV5D HA-PPM1H S211A | Mammalian expression of HA-tagged human PPM1H S211A |
| 208370 | pCMV5D HA-PPM1H S124A/S211A | Mammalian expression of HA-tagged human PPM1H S124A/S211A |