Glutamate indicators with increased sensitivity and tailored deactivation rates
Aggarwal A, Negrean A, Chen Y, Iyer R, Reep D, Liu A, Palutla A, Xie ME, MacLennan BJ, Hagihara KM, Kinsey LW, Sun JL, Yao P, Zheng J, Tsang A, Tsegaye G, Zhang Y, Patel RH, Arthur BJ, Hiblot J, Leippe P, Tarnawski M, Marvin JS, Vevea JD, Turaga SC, Tebo AG, Carandini M, Rossi LF, Kleinfeld D, Konnerth A, Svoboda K, Turner GC, Hasseman J, Podgorski K
bioRxiv 2025.03.20.643984
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 234435 | pAAV-syn-iGluSnFR4s-PDGFR-WPRE | AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested. |
| 234436 | pAAV-syn-iGluSnFR4f-PDGFR-WPRE | AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested. |
| 234437 | pAAV-syn-iGluSnFR4s-NGR-WPRE | AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested. |
| 234438 | pAAV-syn-iGluSnFR4f-NGR-WPRE | AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested. |
| 234439 | pAAV-syn-flex-iGluSnFR4s-PDGFR-WPRE | AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested. |
| 234440 | pAAV-syn-flex-iGluSnFR4f-PDGFR-WPRE | AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested. |
| 234441 | pAAV-syn-flex-iGluSnFR4s-NGR-WPRE | AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested. |
| 234442 | pAAV-syn-flex-iGluSnFR4f-NGR-WPRE | AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested. |
| 234443 | pRSET-iGluSnFR4s | Bacterial expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow) |
| 234444 | pRSET-iGluSnFR4f | Bacterial expression of glutamate sensor with with improved sensitivity and fast deactivation kinetics (4f = fast) |
| 234445 | pAAV-GFAP-iGluSnFR4s-PDGFR-WPRE | AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested. |
| 234446 | pAAV-GFAP-iGluSnFR4f-PDGFR-WPRE | AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested. |
| 234447 | pAAV-CAG-flex-iGluSnFR4s-PDGFR-WPRE | AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested. |
| 234448 | pAAV-CAG-flex-iGluSnFR4f-PDGFR-WPRE | AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested. |
| 234449 | pAAV-CAG-iGluSnFR4s-PDGFR-WPRE | AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested. |
| 234450 | pAAV-CAG-iGluSnFR4f-PDGFR-WPRE | AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested. |
| 234451 | pAAV-CAG-iGluSnFR4s-NGR-WPRE | AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested. |
| 234452 | pAAV-CAG-iGluSnFR4f-NGR-WPRE | AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested. |
| 234453 | pAAV-syn-FLP-FRT-iGluSnFR4s-NGR-WPRE | AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested. |
| 234454 | pAAV-syn-FLP-FRT-iGluSnFR4f-NGR-WPRE | AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested. |