Glutamate indicators with increased sensitivity and tailored deactivation rates
Abhi Aggarwal, Adrian Negrean, Yang Chen, Rishyashring Iyer, Daniel Reep, Anyi Liu, Anirudh Palutla, Michael E Xie, Bryan MacLennan, Kenta M Hagihara, Lucas W Kinsey, Julianna L Sun, Pantong Yao, Jihong Zheng, Arthur Tsang, Getahun Tsegaye, Yonghai Zhang, Ronak H Patel, Benjamin J Arthur, Julien Hiblot, Philipp Leippe, Miroslaw Tarnawski, Jonathan S Marvin, Jason D Vevea, Srinivas C Turaga, Alison G Tebo, Matteo Carandini, L. Federico Rossi, David Kleinfeld, Arthur Konnerth, Karel Svoboda, Glenn C Turner, Jeremy Hasseman, Kaspar Podgorski
bioRxiv 2025.03.20.643984
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 234435 | pAAV-syn-iGluSnFR4s-PDGFR-WPRE | AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested. |
| 234436 | pAAV-syn-iGluSnFR4f-PDGFR-WPRE | AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested. |
| 234437 | pAAV-syn-iGluSnFR4s-NGR-WPRE | AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested. |
| 234438 | pAAV-syn-iGluSnFR4f-NGR-WPRE | AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested. |
| 234439 | pAAV-syn-flex-iGluSnFR4s-PDGFR-WPRE | AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested. |
| 234440 | pAAV-syn-flex-iGluSnFR4f-PDGFR-WPRE | AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested. |
| 234441 | pAAV-syn-flex-iGluSnFR4s-NGR-WPRE | AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested. |
| 234442 | pAAV-syn-flex-iGluSnFR4f-NGR-WPRE | AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested. |
| 234443 | pRSET-iGluSnFR4s | Bacterial expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow) |
| 234444 | pRSET-iGluSnFR4f | Bacterial expression of glutamate sensor with with improved sensitivity and fast deactivation kinetics (4f = fast) |
| 234445 | pAAV-GFAP-iGluSnFR4s-PDGFR-WPRE | AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested. |
| 234446 | pAAV-GFAP-iGluSnFR4f-PDGFR-WPRE | AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested. |
| 234447 | pAAV-CAG-flex-iGluSnFR4s-PDGFR-WPRE | AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested. |
| 234448 | pAAV-CAG-flex-iGluSnFR4f-PDGFR-WPRE | AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested. |
| 234449 | pAAV-CAG-iGluSnFR4s-PDGFR-WPRE | AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested. |
| 234450 | pAAV-CAG-iGluSnFR4f-PDGFR-WPRE | AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested. |
| 234451 | pAAV-CAG-iGluSnFR4s-NGR-WPRE | AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested. |
| 234452 | pAAV-CAG-iGluSnFR4f-NGR-WPRE | AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested. |
| 234453 | pAAV-syn-FLP-FRT-iGluSnFR4s-NGR-WPRE | AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested. |
| 234454 | pAAV-syn-FLP-FRT-iGluSnFR4f-NGR-WPRE | AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested. |