Nanobody-thioesterase chimeras to specifically target protein palmitoylation.

Kuo CW, Gok C, Fulton H, Dickson-Murray E, Adu S, Gallen EK, Mary S, Robertson AD, Jordan F, Dunning E, Mullen W, Smith GL, Fuller W
Nat Commun. 2025 Feb 7;16(1):1445. doi: 10.1038/s41467-025-56716-x. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
234420	pcDNA5-FRT/TO-APT1-LaG16	Express APT1 with the anti-GFP nanobody LaG16 fused at the C terminus separated by a flexible linker
234421	pcDNA5-FRT/TO-APT2-LaG16	Express APT2 with the anti-GFP nanobody LaG16 fused at the C terminus separated by a flexible linker
234422	pcDNA5-FRT/TO-LaG16-APT2	Express APT2 with the anti-GFP nanobody LaG16 fused at the N terminus
234423	pcDNA5-FRT/TO-LaG16-APT1	Express APT1 with the anti-GFP nanobody LaG16 fused at the N terminus
234424	pcDNA5-FRT/TO-APT1-LaG16-3W	Express APT1 with a mutated non-binding anti-GFP nanobody LaG16 (F30W, T55W, G103W) fused at the C terminus separated by a flexible linker
234425	pcDNA5-FRT/TO-APT2-LaG16-3W	Express APT2 with a mutated non-binding anti-GFP nanobody LaG16 (F30W, T55W, G103W) fused at the C terminus separated by a flexible linker
234426	pcDNA5-FRT/TO-APT2-LAMA-G97	Express APT2 with a C terminal fusion to the GFP enhancer nanobody containing dihydrofolate reductase inserted in the nanobody at position G97
234427	cDNA5-FRT/TO-APT2-LAMA-F98	Express APT2 with a C terminal fusion to the GFP enhancer nanobody containing dihydrofolate reductase inserted in the nanobody at position F98