Aaron Hoskins Lab: Hoskins lab plasmids 2025
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 234111 | ACT1-CUP1 46bp dist | PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone |
| 234112 | ACT1-CUP1 50bp dist | PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone |
| 234113 | pRS416-Syf1 | Syf1 region PCR, restriction digest and ligation into pRS416 backbone |
| 234114 | pRS414-Syf1 | Syf1 region PCR, restriction digest and ligation into pRS414 backbone |
| 234115 | pRS414-Syf1 778 Truncation | Syf1 PCR truncation starting at 778 through the end of the gene |
| 234116 | pRS414-Syf1 634 Truncation | Syf1 PCR truncation starting at 634 through the end of the gene |
| 234117 | pRS314-Cef1-Q193P | Suppressor screen mutant in Cef1 |
| 234118 | pRS314-Cef1-M175I | Suppressor screen mutant in Cef2 |
| 234119 | pRS314-Cef1-A37V | Suppressor screen mutant in Cef3 |
| 234120 | pRS424 Prp8 WT TRP1 | The N-terminus of Prp8 was restored thereby destroying the NheI site and correcting the ORF |
| 234121 | pRS424 Prp8 S1584Y | Suppressor screen mutant in full-length Prp8 on a TRP plasmid |
| 234122 | pRS424 Prp8 S1584F | Suppressor screen mutant in full-length Prp8 on a TRP plasmid |
| 234123 | pRS424 Prp8 V1862L | Suppressor screen mutant in full-length Prp8 on a TRP plasmid |
| 234124 | pRS424 Prp8 G1868R | Suppressor screen mutant in full-length Prp8 on a TRP plasmid |
| 234126 | pRS414-Syf1 817 Truncation | Syf1 PCR truncation starting at 817 through the end of the gene |
| 234127 | ACT1-CUP1 42bp dist | PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone |
| 234128 | ACT1-CUP1 15bp dist | PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone |
| 234129 | ACT1-CUP1 27bp dist | PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone |
| 234130 | ACT1-CUP1 12bp dist | PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone |
| 234131 | ACT1-CUP1 9bp dist | PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone |
| 234133 | pRS414-Fyv6-CFLAG | Restriction digest from pUC18-CFLAG-Fyv6 and ligation into pRS414 |
| 234134 | Fyv6-NFLAG del103-173 | Amino acids 103-173 deleted in Fyv6 with FLAG epitope tag at N-terminus |
| 234135 | Fyv6-NFLAG del134-173 | Amino acids 134-173 deleted in Fyv6 with FLAG epitope tag at N-terminus |
| 234136 | Fyv6-NFLAG del1-51 | First 51 amino acids deleted of Fyv6 with FLAG epitope tag at N-terminus |
| 234137 | Fyv6-NFLAG del1-23 | First 23 amino acids deleted of Fyv6 with FLAG epitope tag at N-terminus |
| 234138 | Fyv6-NFLAG del1-16 | First 16 amino acids deleted of Fyv6 with FLAG epitope tag at N-terminus |
| 234139 | pRS414-NFLAG-Fyv6 | Restriction digest from pUC18-NFLAG-Fyv6 and ligation into pRS414 |
| 234140 | pRS414-Fyv6 | Amplified Fyv6 from gDNA, cut with SalI and NotI, ligated into pRS414 |
| 234141 | pRS416-Fyv6 | Amplified Fyv6 from gDNA, cut with SalI and NotI, ligated into pRS416 |