High-throughput screening of human genetic variants by pooled prime editing.
Herger M, Kajba CM, Buckley M, Cunha A, Strom M, Findlay GM
Cell Genom. 2025 Mar 18:100814. doi: 10.1016/j.xgen.2025.100814.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 234070 | pLenti_hU6-peg(ATP1A1-T804N)_mU6-BsmBI_Puro | For lentiviral expression of user-specified pegRNA in tandem with epegRNA (ATP1A1-T804N) for ouabain co-selection |
| 234071 | pLenti_PEmax-2A-BSD | For lentiviral expression of PEmax with blasticidin resistance |
| 234072 | pLenti_PEmax-2A-MLH1dn-2A-BSD | For lentiviral expression of PEmax with MLH1dn and blasticidin resistance |
| 234073 | pLenti_PE7-2A-BSD | For lentiviral expression of PE7 with blasticidin resistance |