A temperature-inducible protein module for control of mammalian cell fate.

Benman W, Huang Z, Iyengar P, Wilde D, Mumford TR, Bugaj LJ
Nat Methods. 2025 Jan 23. doi: 10.1038/s41592-024-02572-4. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
234060	pHR_CMV_Melt-37-mCherry	Encodes a Melt variant with a switching temperature of 37°C
234061	pHR_CMV_Melt-30-mCherry	Encodes a Melt variant with a switching temperature of 30°C
234062	pHR_CMV_Melt-32-mCherry	Encodes a Melt variant with a switching temperature of 32°C
234064	pHR_CMV_MeltTEVp-40-mCherry	Encodes a TEV protease controlled by a Melt variant with a switching temperature of 40°C
234065	pHR_CMV_MeltEGFR-37-mCherry	Encodes EGFR intracellular domain controlled by a Melt variant with a switching temperature of 37°C
234066	pHR_CMV_MeltNLS/NES-40-mCherry	Encodes a protein that localizes to nucleus under the control of a Melt variant with a switching temperature of 40°C
234068	pLV-PGK-TetOn-Puro_MeltITSN1-37-mch	Encodes the Dbl homology and pleckstrin homology (DH-PH) domain of Intersectin1 controlled by a Melt variant with a switching temperature of 37°C
234069	pHR_CMV_MeltCasp1-37-GFP	Encodes the caspase1 without CARD domain (with R391K mutation) controlled by a Melt variant with a switching temperature of 37°C