Measuring pH in insulin secretory granules by phasor-based fluorescence lifetime imaging of a genetically encoded sensor.
De Lorenzi V, Ghignoli S, Bernardi M, Matteoli G, Ferri G, Storti B, Bizzarri R, Cardarelli F
Commun Biol. 2025 Feb 25;8(1):304. doi: 10.1038/s42003-025-07758-w.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 233884 | pCpep-E1GFP | Expression of a pH sensitive variant of EGFP inserted in the C-peptide of insulin for pH measurement in insulin secretory granules |
| 233885 | pET-His-E1GFP | Bacterial Expression of recombinant His-tagged E1GFP |