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Dual targeting of DDX3 and eIF4A by the translation inhibitor rocaglamide A.

Chen M, Asanuma M, Takahashi M, Shichino Y, Mito M, Fujiwara K, Saito H, Floor SN, Ingolia NT, Sodeoka M, Dodo K, Ito T, Iwasaki S
Cell Chem Biol. 2021 Apr 15;28(4):475-486.e8. doi: 10.1016/j.chembiol.2020.11.008. Epub 2020 Dec 8. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
233618pColdI-DDX3_helicase_coreExpression of His-tagged helicase core region of DDX3X in E.coli
233619pColdI-DDX3_helicase_core_Q360LExpression of His-tagged helicase core region of DDX3X with Q360L mutation in E.coli
233620pColdI-DDX3_helicase_core_Q360PExpression of His-tagged helicase core region of DDX3X with Q360P mutation in E.coli
233621pColdI-SBP-eIF4A1Expression of SBP-tagged eIF4A1 in E.coli
233622pColdI-SBP-DDX3_helicase_coreExpression of SBP-tagged helicase core region of DDX3X in E.coli

Antibodies from Article