Simultaneous detection of membrane contact dynamics and associated Ca(2+) signals by reversible chemogenetic reporters.
Garcia Casas P, Rossini M, Pavenius L, Saeed M, Arnst N, Sonda S, Fernandes T, D'Arsie I, Bruzzone M, Berno V, Raimondi A, Sassano ML, Naia L, Barbieri E, Sigismund S, Agostinis P, Sturlese M, Niemeyer BA, Brismar H, Ankarcrona M, Gautier A, Pizzo P, Filadi R
Nat Commun. 2024 Nov 12;15(1):9775. doi: 10.1038/s41467-024-52985-0.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 233594 | OMM-short-RspA-CFAST | Expression of RspA-CFAST on the outer mitochondrial membrane |
| 233595 | ER-RspA-NFAST | Expression of RspA-NFAST on the ER membrane |
| 233596 | ER-NFAST | Expression of NFAST on the ER membrane |
| 233597 | OMM-short-CFAST10 | Expression of CFAST10 on the OMM membrane after a short linker |
| 233598 | OMM-long-CFAST10 | Expression of CFAST10 on the OMM membrane after a long linker |
| 233599 | ER-frNFAST | Expression of frNFAST on the ER membrane |
| 233600 | ER-long-RspA-NFAST | Expression of RspA-NFAST on the ER membrane after a 55 aminoacid linker |
| 233601 | PM-RspA-CFAST | Expression of RspA-CFAST on the PM facing the cytosol |
| 233602 | PM-frNFAST | Expression of frNFAST on the PM facing the cytosol |
| 233603 | OMM-short-RspA-NFAST | Expression of RspA-NFAST on the outer mitochondrial membrane |
| 233604 | ER-mit RspA-splitFAST (lentiviral plasmid) | Expression of ER-mit RspA-splitFAST by a single lentiviral plasmid encoding the two fragments |
| 233606 | STIM1-RspA-NFAST | Expression of hSTIM1-RspA-NFAST |
| 233607 | ER-RspA-CFAST | Expression of RspA-CFAST on the ER membrane |