Rational engineering of allosteric protein switches by in silico prediction of domain insertion sites
Wolf B, Shehu P, Brenker L, von Bachmann A, Kroell A-S, Southern N, Holderbach S, Eigenmann J, Aschenbrenner S, Mathony J, Niopek D
bioRxiv 2024.12.04.626757
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 231574 | PAC_E83_LOV_eGFP | This plasmid mediates puromycin resistance in the dark, resulting in puro mediated cell death upon illumination with blue light |
| 231576 | dSpyCas9-E566-GS-LOV2-VPR | Potent light-deactivated CRISPRa |
| 231577 | MbCas12a_K487_GPG_GR2 | Encodes a cortisol inducible variant of MbCas12a |
| 231578 | MbCas12a_N1153_GPG_GR2 | Encodes a cortisol inducible variant of MbCas12a |