Post-transcriptional modular synthetic receptors.
Zhang X, Mille-Fragoso LS, Kaseniit KE, Lee AP, Zhang M, Call CC, Hu Y, Xie Y, Gao XJ
Nat Chem Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 233374 | EK0450 SFFV-mCherry-SGc(stem-1(3xMS2))cSG-EGFP | LIDAR reporter RNA; mCherry as marker, EGFP as output |
| 233375 | EK0329 CMV-TO-FRB-MCP (FLP-IN) | FRB fused with MCP |
| 233376 | XZ135 CMV-TO-FKBP-ADARdd(QA) (FLP-IN) | FKBP fused with ADAR2dd, with E488Q and T501A double mutation |
| 233377 | YH038 PGK-TO-FRB-tm-MCP | PGK promoter driven FRB fused with MCP, with a CD28 transmembrane domain in between |
| 233378 | YH039 PGK-TO-FKBP-tm-GS-XTEN-ADAR2(DD-E488Q-T501A) | PGK promoter driven FKBP fused with ADAR2dd (E488Q,T501A), with a CD28 transmembrane domain in between |
| 233379 | XZ024 CMV-TO-bArrestin2-MCP (FLP-IN) | ARRB2-tdMCP with a short linker |
| 233380 | XZ030 CMV-TO-AVPR2-ADAR2(DD-E488Q-T501A) (FLP-IN) | AVPR2-V2-ADAR2dd(E488Q, T501A) with a short 4 amino acid linker and extra V2 tail |
| 233381 | XZ362 EF1a-VEGFR2sp-HA-VEGFR2(Ecto+TM)-TS-tdMCP-export | Optimized VEGF-sensing eLIDAR with VEGFR2 ecto+TM domains fused with tdMCP, with Golgi traffic sequence and ER export sequence |
| 233382 | XZ363 EF1a-VEGFR1sp-3xFlag-VEGFR1(ecto+TM)-TS-ADAR2dd(QA)-export | Optimized VEGF-sensing eLIDAR with VEGFR1 ecto+TM domains fused with ADAR2dd, with Golgi traffic sequence and ER export sequence |
| 233383 | YH001 CMV-TO-tm-GS+XTEN-ADAR2(DD-E396A)-RXR (FLP-IN) | ER-retained catalytically inactive ADAR2dd (E396A mutation) |
| 233384 | EK0479 CMV-TO-VEGF-A(165) (FLP-IN) | Secreted human VEGF-A |
| 233385 | XZ377 (PB) EF1a-VEGFR1sp-3xFlag-VEGFR1(ecto+TM)-TS-ADAR2dd(QA)-export-IRESv0-VEGFR2sp-HA-VEGFR2(Ecto+TM)-TS-tdMCP-export | PiggyBac donor plasmid with optimized VEGF-sensing eLIDAR receptor components separated by EMCV IRES, Puromycin resistance and BFP as marker |
| 233386 | XZ375 EF1a-IL2Rbsp-3xFLAG-IL2Rb(ECD+TM)-TS-ADAR2dd-export | Optimized IL-2-sensing eLIDAR with IL-2 beta chain ecto+TM domains fused with ADAR2dd, with Golgi traffic sequence and ER export sequence |
| 233387 | CMVTO-hIL2 | secreted human IL-2 |
| 233388 | XZ393 (PB) EF1a-IL2Rbsp-FLAG-IL2Rb(ECD+TM)-TS-ADAR2dd-export-IRESv0-IL2Rgsp-3xHA-IL2Rg(ECD+TM)-TS-tdMCP-export | PiggyBac donor plasmid with optimized IL-2-sensing eLIDAR receptor components separated by EMCV IRES, Puromycin resistance and BFP as marker |
| 233389 | XZ374 EF1a-IL2Rgsp-3xHA-IL2Rg(ECD+TM)-TS-tdMCP-export | Optimized IL-2-sensing eLIDAR with IL-2 gamma chain ecto+TM domains fused with tdMCP, with Golgi traffic sequence and ER export sequence |
| 233390 | LSM104 CMV-TO-AVPR2-(noExtraV2)-ADAR2(DD-E488Q-T501A) (FLP-IN) | CCR6-ADAR2dd(E488Q, T501A) with a short 4 amino acid linker and no extra V2 tail |
| 233391 | XZ038 CMV-TO-IGF1R-ADAR2(DD-E488Q-T501A) (FLP-IN) | IGF1R-ADAR2dd(E488Q, T501A) |
| 233392 | XZ039 CMV-TO-Shc1-MCP (FLP-IN) | Shc1-tdMCP |
| 233393 | XZ031 CMV-TO-CCR6-ADAR2dd(E488Q, T501A) | CCR6-ADAR2dd(E488Q, T501A) with a short 4 amino acid linker and no V2 tail |
| 233394 | XZ032 CMV-TO-CCR6-V2-ADAR2dd(E488Q, T501A) | CCR6-V2-ADAR2dd(E488Q, T501A) with a short 4 amino acid linker and V2 tail |
| 233395 | LSM044-3 CMV-TO-CCR7-ADAR2dd(E488Q, T501A) (FLP-IN) | CCR7-ADAR2dd(E488Q, T501A) with a short 4 amino acid linker and no V2 tail |
| 233397 | LSM105 CMV-TO-CX3CR1-(noV2)-ADAR2dd(E488Q, T501A) (FLP-IN) | CX3CR1-ADAR2dd(E488Q, T501A) with a short 4 amino acid linker and no V2 tail |
| 233398 | LSM068 CMV-TO-CX3CR1-ADAR2dd(E488Q, T501A) (FLP-IN) | CX3CR1-V2-ADAR2dd(E488Q, T501A) with a short 4 amino acid linker and V2 tail |
| 233399 | XZ113 CMV-TO-signal-hOXTR-ADAR2dd(DD-E488Q-T501A) (FLP-IN) | human oxytocin receptor-ADAR2dd(E488Q, T501A), no V2 tail |
| 233400 | XZ105 CMV-TO-signal-hOXTR-V2-ADAR2dd(DD-E488Q-T501A) (FLP-IN) | human oxytocin receptor-V2-ADAR2dd(E488Q, T501A) |
| 233401 | XZ142 CMV-TO-signal-B2AR-ADAR2dd(DD-E488Q-T501A) (FLP-IN) | _-2 adrenergic receptor-ADAR2dd(E488Q, T501A), no V2 tail |
| 233402 | XZ110 CMV-TO-signal-B2AR-V2-ADAR2dd(DD-E488Q-T501A) (FLP-IN) | _-2 adrenergic receptor-V2-ADAR2dd(E488Q, T501A) |
| 233403 | LSM060 CMV-TO-AVPR2-(10aa-linker)-MCP (FLP-IN) | AVPR2-V2-tdMCP with a 10 amino acid linker and extra V2 tail |
| 233406 | LSM052 CMV-TO-CCR6-PCP (FLP-IN) | CCR6-V2-PCP with a 4 amino acid linker and V2 tail |
| 233408 | LSM041-3 SFFV-mCherry-SGc(stem-1(3xPP7))cSG-EGFP | LIDAR reporter RNA with PCP binding site instead of MCP; mCherry as marker, EGFP as output |
| 233409 | LSM053 SFFV-mCherry-SGc(stem-1(3xPP7))cSG-mtagBFP (FLP-IN) | LIDAR reporter RNA with PCP binding site instead of MCP; mCherry as marker, mTagBFP as output |
| 233410 | APL0038 Ac5-mCherry-SGc(stem-1(3xMS2))cSG-EGFP | AC5 promoter driven LIDAR reporter RNA for drosophila cell expression (mCherry as marker, EGFP as output) |
| 233411 | APL0040 Ac5-FKBP-ADARdd | AC5 promoter driven FRBP-ADAR2dd for drosophila cell expression |
| 233412 | APL0041 Ac5-FRB-MCP | AC5 promoter driven FRB-MCP for drosophila cell expression |
| 233413 | LSM043_2 (PB)SFFV-LIDARSensor(mCherry->Casp9-2A-GFP)-hygro | LIDAR reporter RNA; mCherry as marker, iCasp-P2A-EGFP as output |
| 233414 | XZ400 SFFV-mCherry-SGc(stem-1(3xMS2))cSG-TEVP (equivalent to EK0461) | LIDAR reporter RNA; mCherry as marker, TEVP as output (equivalent to EK0461) |
| 233415 | XZ392 SFFV-secNLuc-SGc(stem-1(3xMS2))cSG(tight)-ssSEAP | LIDAR reporter RNA; secreted NanoLuc Luciferase as marker, SEAP as output |
| 233416 | XZ034 CMV-TO-CCL20 | plasmid expressing secreted soluble CCL20 |
| 233417 | XZ033 CMV-TO-CCL20-pre-mGRASP | plasmid expressing membrane-bound CCL20 by fusing with pre-mGRASP |
| 233419 | XZ054 (PB)SFFV-LIDARsensor (hygR) | PiggyBac donor plasmid with SFFV driven LIDAR reporter RNA (mCherry as marker, EGFP as output) for stable integration |
| 233420 | XZ073 (IVT) CMV-TO-T7-LIDARsensor-hybridUTR3 | IVT template for LIDAR reporter mRNA; mCherry as marker, EGFP as output |
| 233421 | XZ088 (IVT) CMV-TO-T7-CCR6-V2-ADAR2dd-hybridUTR3 | IVT template for CCR6-V2-ADAR2dd(E488Q, T501A) |
| 233422 | XZ089 (IVT) CMV-TO-T7-ARRB2-MCP-hybridUTR3 | IVT template for ARRB2-tdMCP |
| 233423 | XZ387 (IVT) CMV-TO-T7-AVPR2-V2-ADAR2dd-hybridUTR3 | IVT template for AVPR2-V2-ADAR2dd(E488Q, T501A) |
| 233424 | XZ388 (IVT) CMV-TO-T7-EGFP-SGc(stem-1(3xMS2))cSG-Cre-hybridUTR3 | IVT template for LIDAR reporter mRNA; EGFP as marker, Cre recombinase as output |
| 233425 | XZ390 (IVT) CMV-TO-T7-mCherry-SGc(stem-1(3xMS2))cSG-rTetR-NZF-NLS-hybridUTR3 | IVT template for LIDAR reporter mRNA; mCherry as marker, rtetR-NZF as output |
| 233426 | LSM042 (PB)EF1a-AVPR-ADARdd-P2A-PuroR-IRES-ARRB2-MCP | PiggyBac donor plasmid AVPR2-V2-ADAR2dd(E488Q,T501A) and ARRB2-tdMCP separated by EMCV IRES |
| 233427 | LSM061 (PB)SFFV-LIDARsensor(mcherry-BFP) (hygR) | PiggyBac donor plasmid with SFFV driven LIDAR reporter RNA (mCherry as marker, TagBFP as output) |
| 233428 | XZ087 SFFV-mCherry-sensor(tight)-hIgG1-TM | LIDAR reporter RNA with a membrane-bound human IgG as output |
| 233429 | XZ098 SFFV-mCherry-LIDARsensor(W)-hIgG1-TM | Positive control LIDAR reporter RNA (UAG stop codon replaced with UGG) with a membrane-bound human IgG as output |
| 233430 | XZ093 SFFV-sensor-IRESv0-LIDARst(IRESv0-AVPR2) | LIDAR all components on single transcript (as shown in Figure 4A), using EMCV IRES to separate each component |
| 233431 | YX022 (PB)EF1a-LIDARsensor (hygR) | EF1a-driven LIDAR reporter RNA |
| 233432 | LSM101 CMV-TO-ADAR2dd(E488Q,T501A)-MCP | ADAR2dd(E488Q,T501A)-MCP (positive control that fuses ADAR2dd and MCP on the same protein) |
| 233433 | XZ076 (PB) CMV-mCCL20 (PuroR-BFP) | PiggyBac donor plasmid, membrane-bound CCL20 |
| 233434 | XZ091 (PB) CMV-sCCL20 (PuroR-BFP) | PiggyBac donor plasmid, secreted CCL20 |
| 233435 | XZ079 (PB)EF1a-CCR6-ADARdd-P2A-PuroR-IRES-ARRB2-MCP | PiggyBac donor plasmid with CCR6-V2-ADAR2dd(E488Q,T501A) and ARRB2-tdMCP separated by EMCV IRES |
| 233436 | APL0030 SFFV-bArrestin2-MCP (FLP-IN) | SFFV driven ARRB2-tdMCP with a short linker |
| 233437 | APL0031 SFFV-AVPR2-ADAR2dd (FLP-IN) | SFFV driven AVPR2-ADAR2dd (as in XZ030) |
| 233438 | APL0043 SFFV-CCR6-ADAR2dd (FLP-IN) | SFFV driven CCR6-ADAR2dd (as in XZ032) |
| 233439 | LSM075 SFFV-mCherry-SGc(stem-1(3xMS2)x2)cSG-EGFP (FLP-IN) | SFFV driven LIDAR reporter with 2 tandem substrate stem loops |
| 233440 | LSM081 SFFV-mCherry-SGc(stem-1(3xMS2)2-stops)cSG-EGFP (FLP-IN) | SFFV driven LIDAR reporter with an additional stop codon on the stem loop |
| 233441 | LSM0250 (PB)EF1a-CCR6V2-ADAR2dd-IRESv0-PuroR-P2A-BFP | PiggyBac donor plasmid to overexpress CCR6-ADAR2dd (gLIDAR) |
| 233442 | LSM0251 (PB)EF1a-FKBP-tm-ADAR2ddIRESv0-PuroR-P2A-BFP | PiggyBac donor plasmid to overexpress FKBP-TM-ADAR2dd (eLIDAR) |
| 233444 | LSM0253 (PB)EF1a-ADAR2dd-MCP-T2A-PuroR-P2A-BFP | PiggyBac donor plasmid to overexpress ADAR2dd-MCP |
| 233445 | LSM0254 (PB)EF1a-ADAR2-T2A-PuroR-P2A-BFP | PiggyBac donor plasmid to overexpress a full length ADAR2 |
| 233446 | LSM0258 (PB)EF1a-ADAR2dd-PCP-T2A-PuroR-P2A-BFP | PiggyBac donor plasmid to overexpress ADAR2dd-PCP |