Molecular recording of cellular protein kinase activity with chemical labeling
Sun D, Wang Ng S, Zheng Y, Xie S, Schwan N, Breuer P, Hoffmann DC, Michel J, Azorin DD, Boonekamp KE, Winkler F, Wick W, Boutros M, Li Y, Johnsson K
bioRxiv 2024.09.11.611894
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 233353 | pCDNA5/FRT_CMV_NES-Kinprola_PKA-mEGFP | CMV driven expression of the PKA activity recorder Kinprola_PKA in mammalian cell lines fused to mEGFP |
| 233354 | pCDNA5/FRT_CMV_NES-Kinprola_PKA_T/A-mEGFP | CMV driven expression of the inactive PKA activity recorder negative control with T/A mutation Kinprola_PKA_T/A in mammalian cell lines fused to mEGFP |
| 233355 | pCDNA5/FRT_CMV_NES-Kinprola_on-mEGFP | CMV driven expression of the consitutively active kinase activity recorder positive control Kinprola_on in mammalian cell lines fused to mEGFP |
| 233356 | pCDNA5/FRT_CMV_NES-Kinprola_off-mEGFP | CMV driven expression of the inactive kinase activity recorder negative control Kinprola_off in mammalian cell lines fused to mEGFP |
| 233357 | pCDNA5/FRT_CMV_NES-Kinprola_PKC-mEGFP | CMV driven expression of the PKC activity recorder Kinprola_PKC in mammalian cell lines fused to mEGFP |
| 233358 | pCDNA5/FRT_CMV_NES-Kinprola_PKC_T/A-mEGFP | CMV driven expression of the inactive PKC activity recorder negative control with T/A mutation Kinprola_PKC_T/A in mammalian cell lines fused to mEGFP |
| 233359 | pCDNA5/FRT_CMV_NES-Kinprola_JNK-mEGFP | CMV driven expression of the JNK activity recorder Kinprola_JNK in mammalian cell lines fused to mEGFP |
| 233360 | pCDNA5/FRT_CMV_NES-Kinprola_JNK_T/A-mEGFP | CMV driven expression of the inactive JNK activity recorder negative control with T/A mutation Kinprola_JNK_T/A in mammalian cell lines fused to mEGFP |
| 233361 | pCDNA5/FRT_CMV_NES-Kinprola_AMPK-mEGFP | CMV driven expression of the AMPK activity recorder Kinprola_AMPK in mammalian cell lines fused to mEGFP |
| 233362 | pCDNA5/FRT_CMV_NES-Kinprola_AMPK_T/A-mEGFP | CMV driven expression of the inactive AMPK activity recorder negative control with T/A mutation Kinprola_AMPK_T/A in mammalian cell lines fused to mEGFP |
| 233363 | pCDNA5/FRT_CMV_Kinprola_PKA-mEGFP-MTBD | CMV driven expression of the PKA activity recorder Kinprola_PKA in mammalian cell lines fused to mEGFP, targeting to the microtubule |
| 233364 | pAAV_CAG_NES-Kinprola_PKA-mTagBFP2_WPRE-SV40 | CAG driven expression of the PKA activity recorder Kinprola_PKA fused to mTagBFP2 for mammalian cell expression through AAV transduction |
| 233365 | pAAV_CAG_Kinprola_PKA-mEGFP-NLS3__WPRE-SV40 | CAG driven expression of the PKA activity recorder Kinprola_PKA fused to mEGFP for mammalian cell expression through AAV transduction |
| 233367 | pAAV_hSyn_NES-Kinprola_PKA_T/A-mEGFP_WRPE-SV40 | hSyn1 driven expression of the inactive PKA activity recorder negative control with T/A mutation Kinprola_PKA fused to mEGFP for neuronal expression through AAV transduction |
| 233368 | pAAV_hSyn_NES-Kinprola_on-mEGFP_WRPE-SV40 | hSyn1 driven expression of the consitutively active kinase activity recorder positive control Kinprola_on fused to mEGFP for neuronal expression through AAV transduction |
| 233369 | pLKO.1-puro_CMV_NES-Kinprola_PKA-mEGFP | CMV driven expression of the PKA activity recorder Kinprola_PKA fused to mEGFP for neuronal expression through lentivirus transduction |
| 233370 | pLKO.1-puro_CMV_NES-Kinprola_on-mEGFP | CMV driven expression of the consitutively active kinase activity recorder positive control Kinprola_on fused to mEGFP for neuronal expression through lentivirus transduction |
| 233371 | pLenti_EF1α-NES-Kinprola_PKA-mEGFP_bGH-PA term_EF1α_FLAG-SV40NLS-Cas9-NLS-T2A-BSD-WPRE-SV40 | EF1α driven co-expression of the PKA activity recorder Kinprola_PKA fused to mEGFP and Cas9 expression through lentivirus transduction |
| 233372 | pLenti_EF1α-NES-Kinprola_PKA_T/A-mEGFP_bGH-PA term_EF1α_FLAG-SV40NLS-Cas9-NLS-T2A-BSD-WPRE-SV40 | EF1α driven co-expression of the inactive PKA activity recorder negative control with T/A mutation Kinprola_PKA_T/A fused to mEGFP and Cas9 expression through lentivirus transduction |
| 233373 | pLenti_EF1α-NES-Kinprola_on-mEGFP_bGH-PA term_EF1α_FLAG-SV40NLS-Cas9-NLS-T2A-BSD-WPRE-SV40 | EF1α driven co-expression of the consitutively active kinase activity recorder positive control Kinprola_on fused to mEGFP and Cas9 expression through lentivirus transduction |