Isogenic comparison of Airn and Xist reveals core principles of Polycomb recruitment by lncRNAs.
Trotman JB, Abrash EW, Murvin MM, Braceros AK, Li S, Boyson SP, Salcido RT, Cherney RE, Bischoff SR, Kaufmann K, Eberhard QE, Zhang Z, Cowley DO, Calabrese JM
Mol Cell. 2025 Mar 20;85(6):1117-1133.e14. doi: 10.1016/j.molcel.2025.02.014.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 233264 | pCARGO-RMCE | Backbone plasmid used to clone lncRNA inserts in preparation for RMCE |
| 233265 | pOG-Cre | Plasmid expressing Cre recombinase that is used to insert DNA CARGO into RMCE homing locus in Rosa26 |
| 233266 | pCARGO-RMCE-Airn | CARGO plasmid containing ~90kb dox-inducible Airn transgene |
| 233268 | pCARGO-RMCE-Xist | CARGO plasmid containing ~22kb dox-inducible Xist transgene |