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Compact transcription factor cassettes generate functional, engraftable motor neurons by direct conversion

Wang, Nathan B. et al.
Cell Systems, Volume 0, Issue 0, 101206 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
233185pMXs-HRAS(G12V)-IRES-p53DDRetroviral expression of HRAS(G12V) and p53DD (RIDD)
233186pENTR-HRAS(G12V)-IRES-p53DDGateway entry vector for HRAS(G12V) and p53DD (RIDD)
233187pMXs-p53DD-IRES-HRAS(G12V)Retroviral expression of p53DD and HRAS(G12V) (DDIR)
233188pENTR-p53DD-IRES-HRAS(G12V)Gateway entry vector for p53DD and HRAS(G12V) (DDIR)
233189pMXs-mNgn2-x3HARetroviral expression of tagged mNgn2x3HA
233190pMXs-mNgn2-x3FLAGRetroviral expression of tagged mNgn2x3FLAG
233191pMXs-mNgn2-x1HARetroviral expression of tagged mNgn2x1HA
233192pMXs-NIL (with mNgn2x3HA)Retroviral expression of Ngn2x3HA, Isl1, and Lhx3 for motor neuron reprogramming
233193pMXs-NLI (with mNgn2x3HA)Retroviral expression of Ngn2x3HA, Lhx3, and Isl1 for motor neuron reprogramming
233194pMXs-LIN (with mNgn2x3HA)Retroviral expression of Lhx3, Isl1, and Ngn2x3HA for motor neuron reprogramming
233195pMXs-LNI (with mNgn2x3HA)Retroviral expression of Lhx3, Ngn2x3HA, and Isl1 for motor neuron reprogramming
233196pMXs-ILN (with mNgn2x3HA)Retroviral expression of Isl1, Lhx3, and Ngn2x3HA for motor neuron reprogramming
233197pMXs-INL (with mNgn2x3HA)Retroviral expression of Isl1, Ngn2x3HA, and Lhx3 for motor neuron reprogramming
233198pMXs-SNAP-p53DD-IRES-HRAS(G12V)Retroviral expression of SNAP-p53DD and HRAS(G12V)
233199pENTR-HRAS(G12V)-IRES-SNAP-p53DDGateway entry vector for HRAS(G12V) and SNAP-p53DD
233201pENTR-SNAP-p53DD-IRES-HRAS(G12V)Gateway entry vector for SNAP-p53DD and HRAS(G12V)
233202pMXs-rtTARetroviral expression of rtTA for dox-inducible activation from a TRE promoter
233203pAAV-CMV-LNI-bGHAAV expression of Lhx3, Ngn2x3HA, and Isl1 for motor neuron reprogramming
233204pAAV-CAG-SNAP-p53DD-IRES-HRAS(G12V)-bGHAAV expression of SNAP-p53DD and HRAS(G12V)
233205pFUW-LNILentiviral dox-inducible expression of Lhx3, Ngn2x3HA, and Isl1 for motor neuron reprogramming
233206pFUW-SNAP-p53DD-IRES-HRAS(G12V)Lentiviral dox-inducible expression of SNAP-p53DD and HRAS(G12V)
233207pMXs-jRCaMP1bRetroviral expression of jRCaMP1b calcium reporter

Antibodies from Article