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Proliferation history and transcription factor levels drive direct conversion.

Wang NB, Lende-Dorn BA, Adewumi HO, Beitz AM, Han P, O'Shea TM, Galloway KE
Cell Systems, Volume 0, Issue 0, 101205 (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
233148pMXs-mNgn2Retroviral expression of Ngn2
233149pMXs-Isl1Retroviral expression of Isl1
233150pMXs-mLhx3Retroviral expression of Lhx3
233151pMXs-mBrn2Retroviral expression of Brn2
233152pMXs-mAscl1Retroviral expression of Ascl1
233153pMXs-hMyt1lRetroviral expression of Myt1l
233154pMXs-NILRetroviral expression of Ngn2, Isl1, and Lhx3 for motor neuron reprogramming
233155pMXs-mRuby2Retroviral expression of fluorescent mRuby2 reporter
233156pMXs-mBrn2-P2A-mRuby2Retroviral expression of fluorescent mRuby2 reporter
233157pENTR-mNgn2x3HA-P2A-mRuby2Retroviral expression of Ngn2x3HA with mRuby2 fluorescent reporter
233158pENTR-Isl1-P2A-mRuby2Retroviral expression of Isl1 with mRuby2 fluorescent reporter
233159pENTR-mLhx3-P2A-mRuby2Retroviral expression of mLhx3 with mRuby2 fluorescent reporter
233160pMXs-Isl1-T2A-mLhx3Retroviral expression of Isl1 and Lhx3 for motor neuron specification
233162pMXs-mNgn2x3HA-T2A-mLhx3Retroviral expression of mNgn2x3HA and Lhx3
233163pMXs-mNgn2x3HA-P2A-mRuby2Retroviral expression of Ngn2x3HA with mRuby2 fluorescent reporter
233164pMXs-uORFwx1-mNgn2x3HA-P2A-mRuby2Retroviral expression of Ngn2x3HA with mRuby2 fluorescent reporter with upstream open reading frame
233165pMXs-uORFx1-mNgn2x3HA-P2A-mRuby2Retroviral expression of Ngn2x3HA with mRuby2 fluorescent reporter with upstream open reading frame
233166pMXs-uORFx2-mNgn2x3HA-P2A-mRuby2Retroviral expression of Ngn2x3HA with mRuby2 fluorescent reporter with upstream open reading frame
233167pMXs-uORFx3-mNgn2x3HA-P2A-mRuby2Retroviral expression of Ngn2x3HA with mRuby2 fluorescent reporter with upstream open reading frame
233168pMXs-uORFx6-mNgn2x3HA-P2A-mRuby2Retroviral expression of Ngn2x3HA with mRuby2 fluorescent reporter with upstream open reading frame
233169pMXs-Isl1-P2A-mRuby2Retroviral expression of Isl1 with mRuby2 fluorescent reporter
233170pMXs-uORFwx1-Isl1-P2A-mRuby2Retroviral expression of Isl1 with mRuby2 fluorescent reporter with upstream open reading frame
233171pMXs-uORFx1-Isl1-P2A-mRuby2Retroviral expression of Isl1 with mRuby2 fluorescent reporter with upstream open reading frame
233172pMXs-uORFx2-Isl1-P2A-mRuby2Retroviral expression of Isl1 with mRuby2 fluorescent reporter with upstream open reading frame
233173pMXs-uORFx3-Isl1-P2A-mRuby2Retroviral expression of Isl1 with mRuby2 fluorescent reporter with upstream open reading frame
233174pMXs-uORFx6-Isl1-P2A-mRuby2Retroviral expression of Isl1 with mRuby2 fluorescent reporter with upstream open reading frame
233175pMXs-mLhx3-P2A-mRuby2Retroviral expression of mLhx3 with mRuby2 fluorescent reporter
233176pMXs-uORFwx1-mLhx3-P2A-mRuby2Retroviral expression of mLhx3 with mRuby2 fluorescent reporter with upstream open reading frame
233177pMXs-uORFx1-mLhx3-P2A-mRuby2Retroviral expression of mLhx3 with mRuby2 fluorescent reporter with upstream open reading frame
233178pMXs-uORFx2-mLhx3-P2A-mRuby2Retroviral expression of mLhx3 with mRuby2 fluorescent reporter with upstream open reading frame
233179pMXs-uORFx3-mLhx3-P2A-mRuby2Retroviral expression of mLhx3 with mRuby2 fluorescent reporter with upstream open reading frame
233180pMXs-uORFx6-mLhx3-P2A-mRuby2Retroviral expression of mLhx3 with mRuby2 fluorescent reporter with upstream open reading frame
233181pMXs-HRAS(GV12)Retroviral expression of HRAS(G12V)
233182pMXs-myrAkt-P2A-BCL2Retroviral expression of myristoylated Akt1 (myr-Akt) and Bcl2
233183pMXs-cMycRetroviral expression of c-Myc
233184pMXs-hNeuroD1Retroviral expression of NeuroD1

Antibodies from Article